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松材线虫快速检测试剂盒研制

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2007年04期
Page:
133-135
Column:
研究论文
publishdate:
2007-04-20

Article Info:/Info

Title:
Development of Quick Detection Kit for Bursaphelenchus xylophilus
Article ID:
1000-2006(2007)04-0133-03
Author(s):
CHEN Feng-mao12 YE Jian-ren1* WU Xiao-qin1 TANG Jian2
1. College of Forest Resources and Environment Nanjing Forestry University, Nanjing 210037, China; 2. Anhui Forestry Department, Hefei 230031, China
Keywords:
Bursaphelenchus xylophilus Quick detection Kit
Classification number :
S763
DOI:
10.3969/j.jssn.1000-2006.2007.04.030
Document Code:
A
Abstract:
Bursaphelenchus xylophilus, B. mucronatus and the other nematodes separated from dead pine. were detected by using primer K09F2/R. The results indicated that the PCR product of all B. xylophilus isolates had a clear and bright fragment about 340bp. But B. mucronatus and other nematodes had not any fragments. So pairwise K09 F2/R was used for constructing identification kit of B. xylophilus. It took a short time to identify nematodes by adopting this kit. The time of total detection process was within two and half hours. Besides, the sensitivity of this kit reached to 1/7 of a nematode. The detection result is easy to estimate, and the cost of detection is quite low. This kit is a rapid, reliable and convenient means for detecting B. xylophilus.

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Last Update: 2013-05-20