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松树寄生线虫的收集与保存

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2007年04期
Page:
136-138
Column:
研究论文
publishdate:
2007-04-20

Article Info:/Info

Title:
Collection and Conservation of Nematode Germplasm in Pine
Article ID:
1000-2006(2007)04-0136-03
Author(s):
PAN Hong-yang12 YE Jian-ren1* CHEN Feng-mao13 HUANG Ren-e1
1. College of Forest Resources and Environment Nanjing Forestry University, Nanjing 210037, China; 2. The General Station of Forest Pest Control, State Administration of Forestry, Shenyang 110034, China; 3. Anhui General Station of Forest Pest Management, Hefei 230031, China
Keywords:
Pine Nematode Germplasm Collection Conservation
Classification number :
S763
DOI:
10.3969/j.jssn.1000-2006.2007.04.031
Document Code:
A
Abstract:
There are lots of species of nematodes in a dead pine. However we did not know the pathogenicity of these nematodes to pine except Bursaphelenchus xylophilus and B. mucronatus. It is a basic work for study to collect and conserve these nematodes in pine. In this study, 212 nematode isolates had been collected, which were B. xylophilus, B. mucronatus、B. hofmanni, Aphelenchoides menthae, A. macronucleatus, Seinura sp. and Ektaphelenchidae sp. based on species, and isolates were 171, 22, 5, 1, 8, 3 and 2 respectiVely. Among them, the collection rate was 80.7% of B. xylophilus. According to geographic origins, nematode isolates obtained from China, Japan, America, Canada and Korea were 198, 10, 2, 1 and 1 respectively, and 93.4% nematodes came from China. All the above living nematodes were preserved with Bortrytis cinerea, and the DNA of 173 isolates of B. xylophilus, 5 isolates of B. mucronatus, and 5 isolates of other nematodes were preserved under the condition of -20℃ and -80℃. It was the first time to preserve nematode germplasm in a large scale in China.

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Last Update: 2013-05-20