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松枯梢病拮抗细菌的分离和筛选

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2007年04期
Page:
144-146
Column:
研究简报
publishdate:
2007-04-20

Article Info:/Info

Title:
Isolation and Screen of Antagonistic Bacteria Against Sphaeropsis and Bioassay on Seedling of Pine
Article ID:
1000-2006(2007)04-0144-03
Author(s):
JIANG Ping12 YE Jian-ren1*
1. College of Forest Resources and Environment Nanjing Forestry University, Nanjing 210037, China; 2. Forestry College of Xinjiang Agricultural University, Urumqi 830052, China
Keywords:
Sphaeropsis sapinea Antagonistic bacteria Isolation Screen Bioassay
Classification number :
S763.1
DOI:
10.3969/j.jssn.1000-2006.2007.04.034
Document Code:
A
Abstract:
Through tissue isolation and plate dilution, 891 bacteria isolations were obtained from healthy and diseased leaves of pines. By the dural culture method on PDA plates with pathogens, 16 bacteria isolates had strong antagonism against Sphaeropsis sapinea. Under artificial inoculation 10 representative strains were used on the seedling of pines in green house for identifying their biocontrol effects on S. sapinea. Three (Strain number N29, M (2) 21 and S (1) 102) of 10 isolates had both good roles on PDA plates and seedlings of pine trees. The diameters of their inhabit circle were respectively 28.5, 31, 37.5mm and protective effection to the seedlings of pine was over 60%. The result of pathogenicity test demonstrated that they had no harmful influence to the seedling of pine.

References

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Last Update: 2013-05-20