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萱草属野生资源遗传距离和多态性分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2007年06期
Page:
97-100
Column:
研究论文
publishdate:
2007-06-20

Article Info:/Info

Title:
Analysis of the Inter-species Relationships and Polymorphism on the Hemerocallis by ISSR-PCR Molecular Markers
Article ID:
1000-2006(2007)06-0097-04
Author(s):
ZHU Yun-hua12 SU Qian1 WU Wen-ting3 LI Xia1 SHI Jisen1*
1.College of Forest Resources and Environment Nanjing Forestry University, The Key Laboratory of Forest Genetic and Gene Engineering of the State Forestry Administration and Jiangsu Province, Nanjing 210037, China; 2.College of Horticulture Jinling Institute of Technology, Nanjing 210018, China; 3.College of Art Zhejiang University of Technology, Hangzhou 310004, China
Keywords:
Hemerocallis Inter-simple sequence repeat (ISSR) Relationship Polymorphism
Classification number :
Q949.71+8.23
DOI:
10.3969/j.jssn.1000-2006.2007.06.023
Document Code:
A
Abstract:
The ISSR-PCR marker was used to study relationships and genetic polymorphism for nine species and one variation of Hemerocallis. Ten primers were screened from 123 random primers. A total of 218 DNA fragments were amplified. They were polymorphic. The molecular evidence indicated that different species in the genus were high polymorphism. Nei’similarity coefficients and genetic distances was calculated by TFPGA. The dendrogram of nine species and one variation in the genus can be constructed by UPGMA. The results indicated that the nine species and one variation of the Hemerocallis can be divided into four groups. H. lilioasphode, H. citrina, H. fulva, H. dumortieri, H. fulva var. kwanso were in the first group; The second group was the cluster of H. forrestii, H. plicata and H. minor. The third group was H. flava, the fourth group was H. nana. The genetic distance among the different species ranged from 0.0187 to 0.4941. The closest relationship was H. lilioasphode and H. citrina. The next was H. forrestii with H. plicata and H. minor. H. fulva, H. dumortieri, H. fulva var. kwanso were coming after that. H. nana kept the further genetic distance with other daylily species.

References

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Last Update: 2013-05-20