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纸浆浓度及分批补料对里氏木霉产纤维素酶的影响(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2008年03期
Page:
57-60
Column:
研究论文
publishdate:
2008-05-20

Article Info:/Info

Title:
Effects of pulp concentration and fed manner on cellulase production by. Trichoderma reesei
Article ID:
1000-2006(2008)03-0057-04
Author(s):
RAO Qing-long HANG Zhi-xi LAI Chen-huan YU Shi-yuan*
College of Chemical Engineering Nanjing Forestry University, Nanjing 210037, China
Keywords:
Pulp Trichoderma reesei Cellulase Fed-batch
Classification number :
T9352; Q556
DOI:
10.3969/j.jssn.1000-2006.2008.03.013
Document Code:
A
Abstract:
Different pulp concentration as carbon source for cellulase proc’tuction by Trichoderma reesei was investigated. The best pulp concentration was 12 g/L. After 3 days of cultivation, the filter paper activity, CMCase activity and [3-glucosidase activity were 1.68,0.96 and 0.28 U/mL respectively. Fed-batch manner could significant increase cellulase production by Trichoderma reesei. The fed-batch manner was that initial pulp concentration was 9 g/L and 2 g/L pulp was added to the culture at second, third and fourth day. After 3 days of cultivation, the filter paper activity, CMCase activity and β-glucosidase activity became 2.41,0. 72 and 0.27 U/mL respectively. Compared with batch culture used 15 g/L pulp, the filter paper activity increased 1.8 times.

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Last Update: 2013-05-20