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兔耳风属3个近缘种ISSR分子鉴别(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2008年03期
Page:
139-141
Column:
研究简报
publishdate:
2008-05-20

Article Info:/Info

Title:
ISSR molecular identification for three species in Ainsliaea
Article ID:
1000-2006(2008)03-0139-03
Author(s):
WEN Qiang YE Jin-shan YU Hong JIANG Xiang-mei
Jiangxi Academy of Forestry Sciences, Jiangxi Provincial Bio-tech Key Leg for Plant, Nanchang 330032, China
Keywords:
Ainsliaea fragrans A. rubrifolia A. macroclinidioides iISSR molecular identification
Classification number :
S338; Q943
DOI:
10.3969/j.jssn.1000-2006.2008.03.033
Document Code:
A
Abstract:
Three difficultly-divisional species, i. e., Ainsliaea fragrans, A. macroclinidioides and A. rubrifolia were studied using the ISSR molecular identification system. Twenty primers were screened out from 100 ISSR UBC. They could generate reproducible polymorphic fragments. The particular bands were expanded to make the ISSR fingerprint by primer UBC807, UBC826, UBC834, UBC843, UBC853, and UBC895. It could be used to discriminate three species. Furthermore, according to the alike coefficient UPGMA showed that the re]ative relation of A. fragrans and A. rubrifolia was closer than that of A. fragrans and A. macroclinidioide. The research could be responded for the conservancy and development of the chinese herbal medicine resonrce, and providing a technical support of building up the stable qnality evaluation system of chinese herbal medicine.

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Last Update: 2013-05-20