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几种不同楸树花粉萌发率的测定及花粉超低温保存方法

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2008年05期
Page:
123-126
Column:
研究论文
publishdate:
2008-09-30

Article Info:/Info

Title:
Studies on pollen germination and cryopreservation method in different species of Catalpa
Article ID:
1000-2006(2008)05-0123-04
Author(s):
WANG Gai-ping PENG Fang-ren* XU Tao SHANG Yang-juan ZHANG Chun-xia
College of Forest Resourecs and Environment Nanjing Forestry University, Nanjing 210037. China
Keywords:
Catalpa spp. Pollen viability Cryopreservation
Classification number :
S718
DOI:
10.3969/j.jssn.1000-2006.2008.05.025
Document Code:
A
Abstract:
The pollen viability of five species of Catalp, (Catalpa bungei, C. fargesiif, duclouxii, C. ovata, C. fargesii aud C. speciosa) were studied by germinative method on agar plate. The result indicated that the optimal pollen germinatiou eavironment was 25℃ and 611. The optimal germinative mediums scheme was 10% sucrose, 0.01% boric acid and 1% agar. At the condition of water planting and the room temperature, the rate of germination of pollen was the highest during the first day to the third day. It decreased significantly from the forth day to the end of a week. The rate of pollen germination was the highest and kept stable highest level in cryopreservation and-70℃. It was higher than tha! in-20℃ and 0℃. It was the lowest at the room temperature. After storage in liquid nitrogen, thawing treatment at varied temperate of 35--38℃ for 5rain could greatly increase the viability of pollen.

References

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Last Update: 2013-05-20