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不同碳源条件下里氏木霉纤维降解酶的蛋白组解析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2009年01期
Page:
92-96
Column:
研究论文
publishdate:
2009-01-30

Article Info:/Info

Title:
Proteomic analysis of extracellular cellulolytic enzyme in Trichoderma reesei on different carbon sources
Author(s):
LIU Changqing1XU Lin1OUYANG Jia2DONG Zhengwei2YAN Ming1
1.College of Life Science and Pharmacy, Nanjing University of Technology,Nanjing 210009,China; 2.College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
Keywords:
cellulolytic enzymetwo dimensional electrophoresisTrichoderma reeseicarbon sourcesecretome
Classification number :
Q814
DOI:
10.3969/j.jssn.1000-2006.2009.01.019
Document Code:
A
Abstract:
Two dimensional electrophoresis was used to investigate the expression levels of extracellular cellulolytic enzymes of Trichoderma reesei on different carbon sources. The results showed that very low expression levels of CBHⅡ, AxeI and BXL were detected when glucose or glycerol was employed as carbon source. Moderate expression of CBHⅠ, CBHⅡ, EGⅠ, EGⅢ, BXL, AxeI and AglⅠ was observed when cellobiose was used as carbon source. Cellulose induced abundant expression of CBHⅠ,CBHⅡ,EGⅠ,EGⅡ, EGⅢ, EGIV, ManI, ManII, AxeI, BXL and AglⅠ, which accounted for 61%of the secretome. There was some consistency in cellulase composition between the map of cellulose and cellobiose condition, but higher expression levels and enzyme activities were observed when it was induced by cellulose.

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Last Update: 2009-05-06