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运用MuPS标记识别马尾松抗松材线虫病个体(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2009年03期
Page:
1-4
Column:
研究论文
publishdate:
2009-05-30

Article Info:/Info

Title:
Using MuPS marker technique to identify individual of Pinus massoniana resistance to pine wilt disease
Author(s):
GAO Jingbin12 XU Liuyi2 YE Jianren1* ISODA Keiya3 UBUKATA Masatoshi3
1.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China; 2.Anhui Province Forestry Bureau Resistance Breeding for Pine nematode Centre, Hefei 230031, China; 3.Forest Tree Breeding Center Forestry Research Products Research Institute, Ibaraki 3191301, Japan
Keywords:
Bursaphelenchus xylophilus Pinus massoniana resistance breeding MultiplexPCR of SCAR markers
Classification number :
S763
DOI:
10.3969/j.jssn.1000-2006.2009.03.001
Document Code:
A
Abstract:
Using 14 SCAR marker primers to analyze the resistance seedlings of Pinus massoniana which resist Bursaphelenchus xylophilus. The results indicated that there were 104 different types of MuPS among those 120 samples, which could distinguish 86.6% samples, and 74.2% samples identified completely. Some primers such as Pmscar157, Pmscar007 and Pmscar053 produced more bands, which was 13.1%, 13.3%, 18.4% of the total bands respectively, while the primers Pmscar039 and Pmscar499 produced less band, and they were 0.16% and 0.48% of the total bands respectively. The primers used in this paper could fingerprint well because of high stability and reliability. Therefore, the attained MuPS could be used to identify and manage the resistance individual and the resistance family of P.massoniana.

References

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Last Update: 2009-05-30