我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|Table of Contents|

油茶苯丙氨酸解氨酶基因的cDNA克隆与序列分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2009年04期
Page:
24-28
Column:
研究论文
publishdate:
2009-07-30

Article Info:/Info

Title:
Molecular clone and sequence analysis of phenylalanine ammonia lyase gene from Camellia oleifera
Author(s):
TIAN Xiaoming TAN Xiaofeng* HU Xiaoyi LIU Qiao LUO Qian
The Key Lab of Non Wood Forest Products of Forestry Ministry, Central South University of Forestry and Technology, Changsha 410004, China
Keywords:
Camellia oleifera phenylalanine ammonia lyase (PAL) fulllength cDNA gene cloning sequence analysis
Classification number :
Q785
DOI:
10.3969/j.jssn.1000-2006.2009.04.005
Document Code:
A
Abstract:
PAL (phenylalanine ammonia lyase) is one of the key enzymes in plant secondary metabolism. The fulllength cDNA of PAL was identified from the cDNA library and EST library of Camellia oleifera by the method of molecular cloning in this paper. The results showed that the cDNA of C.oleifera PAL contained 2 118 bp, encoding 706 amino acids, and had a high similarity in the evolution of a high degree of homology with other species of PAL; C.oleifera PAL encoded protein sequence contained the same deamination site and catalytic active site with protein PAL of rice and corn. PAL phylogenetic tree analyses revealed that the C. oleifera PAL had closer relationship with PALs from Shrub plants than from other plants.

References

[1]江昌俊,余有本. 苯丙氨酸解氨酶的研究进展[J]. 安徽农业大学学报,2001,28(4):425-430.
[2]程水源,陈昆松,刘卫红,等. 植物苯丙氨酸解氨酶基因的表达调控与研究进展[J]. 果树学报,2003,20(5):351-357.
[3]张党权,谭晓风,陈鸿鹏. 油茶油脂的生物合成及调控基因的特性[J]. 中南林业科技大学学报,2008,27(10):106-112
[4]张党权,谭晓风,陈鸿鹏,等. 油茶SAD基因的全长cDNA克隆及生物信息学分析[J]. 林业科学2008,44(2):155-160.
[5]胡芳名,谭晓风,石明旺,等. 油茶种子cDNA文库构建[J]. 中南林学院学报,2004,24(5):1-4.
[6]谭晓风,胡芳名,谢禄山,等. 油茶种子EST文库构建及主要表达基因的分析[J]. 林业科学,2006,42(1):43-48.
[7]萨姆布鲁克J,拉塞尔D W. 分子克隆实验指南[M]. 2版. 北京:科学出版社,1999. [8]许锋,陈柳吉,蔡荣,等. 海桐花苯丙氨酸解氨酶的基因克隆与序列分析[J]. 西北农业学报,2008,17(2):218-224.
[9]谭晓风,陈鸿鹏,张党权,等. 油茶FAD2基因全长cDNA的克隆和序列分析[J]. 林业科学,2008,44(5):70-76.
[10]Tan X F, Zhang D Q, Qiu J, et al. Separation and bioinformatic analysis of oleosin genes in seeds of Camellia oleifera[R]. The Third International Forum on PostGenome Technologies, 2005: 90-91.
[11]陈新,万德光,严铸云,等. 银杏苯丙氨酸解氨酶(PAL)的基因克隆[J]. 成都中医药大学学报,2004,27(1)31-34.
[12]王燕,张风霞,朱俊,等. 核桃苯丙氨酸解氨酶的基因克隆与序列分析[J]. 湖北农业科学,2008,22(6):622-626.
[13]KUMAR S, TAMURA K, NEI M. MEGA3. Integrated software for molecular evolutionary genetics analysis and sequence alignment [J]. Briefings in Bioinformatics, 2004, 5(2): 150-163.
[14]陈鸿鹏,谭晓风. 超氧化物歧化酶(SOD)研究综述[J]. 经济林研究,2007,25(1):59-65.
[15]ROSLER J, KREKEL F, AMRHEIN N, et al. Maize phenylalanine ammonialyase has tyrosine ammonialyase activity[J]. Plant Physiol, 1997, 113(1): 175-179.
[16]Pedroso MC, MagalhaesJR, Durzan D. Nitric oxide induces cell death in Taxus cells[J]. Plant Sci, 2000, 157(11): 173-180.
[17]胡孝义,谭晓风,田晓明,等. 油茶种子水通道蛋白CoPIP121的鉴定与分析[J]. 林业科学,2008,44(12):48-57.
[18]胡孝义,谭晓风,张党权,等. 1个油茶Y2SK2 型脱水素的全长cDNA克隆及生理功能的预测[J]. 林业科学,2008,44(10):56-64.

Last Update: 2009-07-30