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汽爆玉米秸秆渣诱导产纤维素酶及其水解特性(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2009年04期
Page:
96-100
Column:
研究论文
publishdate:
2009-07-30

Article Info:/Info

Title:
Characteristics of cellulase inducement and hydrolysis by steam exploded corn stover
Author(s):
OUYANG Jia DONG Zhengwei XIE Zhe LI Xin SONG Xiangyang
College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
Keywords:
steam exploded residue cellulase hydrolysis corn stover
Classification number :
TQ352
DOI:
10.3969/j.jssn.1000-2006.2009.04.020
Document Code:
A
Abstract:
The characteristics of cellulase inducement and hydrolysis by steam exploded corn stover were investigated. Steam explosion could remove hemicellulose from corn stover and the acquired residue was a good inducer for cellulase biosynthesis using Trichoderma reesei NL02. When the concentration of substrate was 10 g/L, the filter paper activity reached 1.90 U/mL after cultivation for seven days. The four produced and the two other enzymes were characterized by various enzyme activity measurements and steam exploded corn stover was hydrolyzed by them. The result showed that the highest sugar yields were obtained with the enzyme induced by steam exploded corn stove after cultivating for five days. When 50 g/L steam exploded residue was treated with the enzyme dosage of 25 U/g, the enzymatic hydrolysis yield was 95.9% for 24 h and the glucose yield was 76.4% for 48 h, and when the substrate concentration was improved to 80 g/L with the dosage of 15 U/g, the hydrolysis yield was 90.1% and the glucose yield was 62.9% for 48 h under the same conditions.

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Last Update: 2009-07-30