我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|Table of Contents|

杨树和杉木茎段组织的冰冻切片技术研究

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2009年06期
Page:
44
Column:
研究论文
publishdate:
2009-11-30

Article Info:/Info

Title:
Cryosection technique developed for stem tissue gene expression research on poplar and Chinese fir
Author(s):
LU Ye XI Mengli ZHENG Jia SHI Jisen*
Key Laboratory of Forest Genetics and Biotechnology Ministry of Education, Nanjing Forestry University, Nanjing 210037, China
Keywords:
Populus Cunninghamia lanceolata(Lamb.)Hook cryosection
Classification number :
S722
DOI:
10.3969/j.jssn.1000-2006.2009.06.010
Document Code:
A
Abstract:
Cryosection technique in woody plant becomes the key technology to investigate tissue differential expression and gene localization on woody plant genomic research. The cryosection protocols were developed on two model woody plants: Chinese fir and poplar. The stem samples were firstly processed using paraformaldehyde, acetone and Carnoy as fixative separately. Liquid Nitrogen can protect the tissue especially the tender one such as tissue culture’s materials. The tissue structure and cell morphology can be kept more properly by paraformaldehyde. By comparison, the cryosection was much better than other treatments, adapted with the 10% sucrose to the sample tissue, which has been fixed by acetone or Carnoy, and which can also be applied to the nucleic acid extraction. The adaptive treatment parameters of Chinese fir and poplar tissue culture seedling were 10% sucrose and then 50—90 seconds in liquid Nitrogen. The dehydration can make the cryosections remained clearer and intact. The tissues, which were more lignified, need to be softened by glycerol. The system established for Chinese fir and poplar stem can also be applied to other plant tissues.

References

[1]朱惠君,杨其昌. 手术中快速冰冻切片及相关问题[J]. 江苏医药, 2005,31(12):953.
[2]陈丹,赵洁. 适合于植物花器官的冰冻切片技术[J]. 武汉植物学研究,2005,23(3):285-290.
[3]Ahram M, Flaig M J, Gillespie J W, et al. Evaluation of ethanolfixed, paraffinembedded tissues for proteomic applications[J]. Proteomics, 2003(3): 413-421.
[4]Burgemeister R, Gangnus R, Haar B, et al. High quality RNA retrieved from samples obtained by using LMPC (laser microdissection and pressure catapulting) technology[J]. Pathology, 2003, 199: 431-436.
[5]Asano T, Masumura T, Kusano H, et al. Construction of a specialized cDNA library from plant cells isolated by laser capture microdissection: toward comprehensive analysis of the genes expressed in the rice phloem[J]. The Plant Journal, 2002, 32:401-408.
[6]Nakazono M, Qiu F, Borsuk L A, et al. Lasercapture microdissection, a tool for the global analysis of gene expression in specific plant cell types: Identification of gene expressed differentially in epidermal cells or vascular tissues of Maize [J]. The Plant Cell, 2003, 15: 583-596.
[7]Casson S, Spencer M, Walker K, et al. Laser capture microdissection for the analysis of gene expression during embryogenesis of Arabidopsis[J]. The Plant Journal, 2005, 42: 111-123.
[8]Nakada M, Komatsu M, Ochiai T, et al. Isolation of MaDEF from Muscari armeniacum and analysis of its expression using laser microdissection [J]. Plant Science, 2006, 170: 143-150.
[9]Spencer M W, Casson S A, Lindsey K. Transcriptional profiling of the Arabidopsis embryo [J]. Plant Physiology, 2007,143: 924-940.
[10]陆叶. 杉木、杨树激光显微切割体系的建立及RNA质量的控制[D]. 南京:南京林业大学, 2007.
[11]康莲娣. 生物电子显微技术[M]. 合肥: 中国科学技术大学出版社, 2003.
[12]蒙艳斌,贺莉萍,王晓. 蔗糖磷酸缓冲液对组织标本中RNA 的保护作用[J]. 医学新知杂志,2004,14(2):89-92.
[13]林月惠,李寒冰,贺新强. 高度木质化材料的冰冻切片技术[J]. 植物学通报, 2001,18(1):118-120.
[14]刘剑锋,程云清,阎秀峰,等. 植物冰冻切片技术的改进[J]. 南京林业大学学报:自然科学版, 2006,30(3):128-130.
[15]刘剑锋,阎秀峰,程云清,等. 冰冻切片技术在高山红景天细胞学研究中的应用[J]. 东北林业大学学报,2006,34(4):118-119.
[16]包翠芬,刘霞,穆长征,等. 冰冻切片几种防冰晶方法的比较[J]. 中国误诊学杂志,2006,6(17):3310-3311.
[17]邵景元,顾诤,陈媛. 合成胶水在微小组织冰冻切片中的应用[J]. 西北国防医学杂志,2002,23(1):67-68.

Last Update: 2009-11-30