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赤松离体培养植株再生体系的建立(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2010年02期
Page:
11-14
Column:
研究论文
publishdate:
2010-03-30

Article Info:/Info

Title:
In vitro plantlet regeneration from seedling explants of Pinus densiflora
Author(s):
ZHU Lihua12 WU Xiaoqin12* DAI Peipei12 ZHANG Hongyan12
1.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China; 2.Jiangsu Key Laboratory for Prevention and Management of Invasive Specie, Nanjing 210037, China
Keywords:
Pinus densiflora bud induction shoot multiplication adventitious root plantlet regeneration
Classification number :
S718; Q948
DOI:
10.3969/j.jssn.1000-2006.2010.02.003
Document Code:
A
Abstract:
A plantlet regeneration protocol was developed for P.densiflora by using Cotyledonhypocotyl explants from 21—28 days old aseptically grown seedlings. The explants were cultured on a GD medium containing 4 mg/L 6BA (6benzylaminopurine) and 0.05 mg/L NAA (αnaphthylacetic acid) for 5 weeks to obtain bud induction. Bud growth was enhanced by the elimination of 6BA and the inclusion of 0.1 mg/L NAA in DCR medium. Shoots elongation was achieved on DCR medium supplemented with 0.5—1.0 g/L activated charcoal (AC). Multiple shoots in subcultures were proliferated on GD medium with 2 mg/L BAP and 0.2 mg/L NAA. Root primordia were induced in 68.4% of shoots after 4 weeks of culture on 1/2 GD medium containing 0.2 mg/L NAA. Roots elongated rapidly after the transmission of rooted plantlets to 1/2 GD medium with 0.5 g/L AC. The rooted plantlets were successfully transferred to cups containing a mixture of vermiculiteperlitesand. The surviving rate was approximate 60% after 12 weeks in the greenhouse.

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Last Update: 2010-05-14