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楸树体细胞胚胎发生的研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2010年02期
Page:
15-18
Column:
研究论文
publishdate:
2010-03-30

Article Info:/Info

Title:
Study on somatic embryogenesis of Catalpa bungei
Author(s):
JIANG Rongcui PENG Fangren* TAN Pengpeng HAO Mingzhuo
College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China
Keywords:
Catalpa bungei C.A.Mey callus somatic embryo genesis
Classification number :
S722; Q948
DOI:
10.3969/j.jssn.1000-2006.2010.02.004
Document Code:
A
Abstract:
The process of somatic embryogenesis (SE) of Catalpa bungei and the effects of different growth regulators on SE were studied with explants of cotyledons and hypocotyls of seedlings. The seedlings were cultured from near matured seeds of C.bungei. The results showed that callus could be induced on each medium in this paper. Callus could be transferred to embryogenic callus on the medium of 1/2MS+0.01 mg/L NAA+1.00 mg/L 6-BA, on which the SE were induced with the highest frequency. The SE were able to proliferate on the medium of 1/2MS0 and 1/2MS+1.00 mg/L 6-BA+0.01 mg/L NAA with high multiplication coefficient.

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Last Update: 2010-05-14