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|Table of Contents|

产单宁酶真菌的筛选及产酶条件(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2010年03期
Page:
6-10
Column:
研究论文
publishdate:
2010-06-29

Article Info:/Info

Title:
Study on screening and induction conditions of the tannase producing fungi
Author(s):
GU Wen YANG Yichen WANG Shifa*
Keywords:
tannase screening fermentation condition Trichoderma viride
Classification number :
TQ437
DOI:
10.3969/j.jssn.1000-2006.2010.03.002
Document Code:
A
Abstract:
CollFiftytwo strains of filamentous fungi were isolated from the fresh stems and/or leaves of 4 plants, and 18 tannase producing strains were obtained through the plate transparent zone method. Subsequently these strains were cultured in the liquid tannaseinducing medium, among which a fungal strain Trichoderma viride (No.MYP07) showed the strongest activity (0.248 μmol/(min·mL)). Through single factor and orthogonal experiments, the optimal fermentation conditions of the strain MYP07 were determined as follows:tannin acid 100 g/L, sucrose 10 g/L, (NH4)2SO4 3 g/L, liquid volume 30 mL (in 250 mL flasks), initial pH 5.0, 30 ℃, 140 r/min for 48 h. In these conditions, the tannase activity of the strain was 0.392 μmol/(min·mL).ege of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China

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Last Update: 2010-06-29