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越橘离体快繁体系的优化(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2010年03期
Page:
168-170
Column:
研究简报
publishdate:
2010-06-29

Article Info:/Info

Title:
The optimization of bilberry rapid micropropagation in vitro culture
Author(s):
DUAN Zuan1 LI Jianhua1 ZHAO Yanyan1 WANG Hongli1* HOU Liqun2
1.Forestry College, Shandong Agricultural University, Tai’an 271018, China; 2.Shandong Academy of Forestry, Jinan 250014, China
Keywords:
Vaccinium ssp. explants rapid micropropagation
Classification number :
Q948
DOI:
10.3969/j.jssn.1000-2006.2010.03.035
Document Code:
A
Abstract:
This paper studied the factors affecting bilberry rapid micropropagation with improved WPM as basic culture medium and stem segment of bilberry as explant in vitro culture. The results showed that initial culture system was quickly created when the explants collected stem segment of bilberry in March was treated in dark at pH 4.2 for 22 h and WPM was modified by adding ZT(1 mg/L) and TDZ(0.2 mg/L). The induced differentiation of bilberry multiple shoot clumps were verified with the improved WPM +ZT (1.5 mg/L) by orthogonal experiment.

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Last Update: 2010-06-29