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中国梨SRNase基因启动子的TAILPCR 克隆及功能预测(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2010年04期
Page:
21-25
Column:
研究论文
publishdate:
2010-08-06

Article Info:/Info

Title:
Clone and functional prediction of SRNase promoter regions in Chinese pear
Author(s):
WUYUNTana1; 2;  LIU Xueying1;  LI Zhenguo3
1.The Key Lab of Nonwood Forest Product of Forestry Ministry, Central South University of Forestry and Technology, Changsha 410004, China; 2.School of Forestry Central South University of Forestry and Technology, Changsha 410004, China; 3.Huanggangliang Forest Farm of Keshiketengqi, Chifeng 025350, China
Keywords:
TAILPCR;  Pyrus;  SRNase;  promoter
Classification number :
S722
DOI:
10.3969/j.jssn.1000-2006.2010.04.005
Document Code:
A
Abstract:
Using TAILPCR, 5′flanking regions of the S13, S12, S21RNase genes with a length of 854 bp, 1 448 bp and 1 137 bp were successfully isolated from ‘Jinhua ’and ‘Maogong’(Pyrus pyrifolia) and ‘Yali’(Pyrus bretschneideri) genomic DNA, the GenBank numbers are HM047239, HM047240 and HM047241. The core promoter regions and some upstream regulatory elements in the three fragments were analyzed using PLACE and PlantCARE software. It is found that all of these genes have the putative TATA box and CAAT box, and the promoters were affected by a variety of conditions as light, ABA, salicylic acid. Alignment analysis for promoter sequences of S13, S12, S21 with 5′flanking sequences of S2, S3, S4, S5 revealed a homologous region of about 200 bp in the upstream sequences of the TATA box in SRNase promoters. Phylogenetic tree constructed by MEG 4.0 suggests that the divergence of SRNase gene was formed before the differentiation of subfamilies.

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Last Update: 2010-08-06