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抗病赤松组培丛生芽低温保存条件研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2010年05期
Page:
7-11
Column:
研究论文
publishdate:
2010-10-08

Article Info:/Info

Title:
Study on cryopreservation of auxiliary buds of resistant Pinus densiflora
Author(s):
ZHANG Hongyan WU Xiaoqin* ZHU Lihua TAN Jiajin
College of Forest Resources and Environment, Nanjing Forestry University, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, Nanjing 210037, China
Keywords:
Pinus densiflora tissue culture auxiliary buds cryopreservation pine wood nematode disease
Classification number :
S718
DOI:
10.3969/j.jssn.1000-2006.2010.05.002
Document Code:
A
Abstract:
The conditions for cryopreservation were studied at storage temperature, storage time, sorbitol contention and the combination of 6BA and NAA levels for better understanding of reservation and effective utilization of resistant Pinus densiflora. The results showed that, when reserved at 17 ℃, survival rate of auxiliary buds could be up to 96.7 %, sorbitol and paclobutrazol could alleviate chilling injury, 0.2 mg/L 6BA+(0.1—0.2) mg/L NAA was the best combination of cryopreservation, which could improve the multiplication coefficient to 5.9—6.0 in the course of recovery growth, and the effects of cryopreservation were greatly different from each other,1#-A, 7#-7,16#-2 were elite genotype. It also provided reference for the reservation of other germplasm resources.

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Last Update: 2010-10-08