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果梅RAPD标记不同电泳指纹比较及(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2010年05期
Page:
29-33
Column:
研究论文
publishdate:
2010-10-08

Article Info:/Info

Title:
Comparison of RAPD fingerprints of fruiting mei generated by different electrophoresis and characterization of the amplified sequences
Author(s):
LI Xiaoying SONG Changnian ZHANG Yanping WANG Yujuan ZHANG Zhen FANG Jinggui*
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
Keywords:
Prunus mume Sieb.et Zucc. RAPD electrophoresis sequence analysis
Classification number :
S722
DOI:
10.3969/j.jssn.1000-2006.2010.05.006
Document Code:
A
Abstract:
PCR amplification was conducted with 11 bp primers by the optimized RAPD protocol and we used agarose and polyacrylamide gel (PAGE) electrophoresis to detect RAPD amplification products from 16 fruiting mei cultivars. The results showed that the total and polymorphic bands of PAGE were 204 and 145, while 91 and 58 on agarose gel. The polygenetic tree also showed that all the 16 cultivars could be distinguished unambiguously and their relationship was revealed according to the fingerprinting profiles obtained by PAGE electrophoresis system. Random cloning and sequencing of 20 amplified fragments showed that all these segments were RAPD products generated by the corresponding primers. Among the sequenced fragments, four sequences were from protein coding regions, suggesting RAPD could randomly amplify both coding and noncoding sequences. Thus, genetic variability of different fruiting mei cultivars could be properly reflected by RAPD marker.

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Last Update: 2010-10-08