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铁线莲‘MultiBlue’体细胞胚诱导和植株再生(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2010年06期
Page:
18-22
Column:
研究论文
publishdate:
2010-11-30

Article Info:/Info

Title:
Embryogenesis and plant regeneration of Clematis ‘MultiBlue’
Author(s):
ZHANG Qixiang12 HU Hengkang3 FANG Yanming1*
1.College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China; 2.School of Forestry and Biotechnology, Zhejiang A & F University, Lin’an 311300, China; 3 College of Landscape Architecture and Art, Jiangxi Agriculture University, Nanchang 330045, China
Keywords:
Clematis ‘MultiBlue’ embryogenesis plant regeneration
Classification number :
S718
DOI:
10.3969/j.jssn.1000-2006.2010.06.005
Document Code:
A
Abstract:
Using stem tip of Clematis ‘MultiBlue’ as explants and 1/2 MS as culture media, the suitable conditions for induction of embryogenic callus and somatic embryos, as well as plant regeneration, were determined. The results indicated that the optimal conditions for induction of embryogenic callus were 1/2 MS supplemented with 1.0 mg/L TDZ, 0.5 mg/L NAA and 2 % scourse plus 0.7 % agar. Explants cultured in this solid medium could form yellow massive or granular callus and produced embryogenic callus after subculture. Suitable media for somatic embryo induction were 1/2 MS added with 0.2 mg/L TDZ and 0.1 mg/L IBA plus 0.5 g/L CH, with an induction rate of 96%. The germination rate of somatic embryos was the highest when the plant growth regulators were 0.2 mg/L 6BA and 0.05 mg/L IBA. The plantlets with root were successfully transplanted to the medium with vermiculite. The survival rate of regeneration plant could be up to at least 80 %.

References

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Last Update: 2010-12-27