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液态混合菌发酵优化纤维素酶组分(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2011年02期
Page:
78-82
Column:
研究论文
publishdate:
2011-03-28

Article Info:/Info

Title:
ptimization of cellulase components by mixed culture of Trichoderma reesei and Aspergillus niger
Author(s):
SONG XiangyangFANG HaoOUYANG JiaLI XinYONG Qiang
Key Laboratory of Forest Genetic and Biotechnology Ministry of Education, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037 China
Keywords:
Trichoderma reesei Aspergillus niger mixed culture cellulase component optimization
Classification number :
TQ925
DOI:
10.3969/j.jssn.1000-2006.2011.02.017
Document Code:
A
Abstract:
Mixed culture of Trichoderma reesei and Aspergillus niger was investigated to optimize the cellulase components in liquidstate fermentation. A mode of delayed inoculation was applied for cocultivation of T. reesei and A. niger. After cultivation of T. reesei for 48 h, A. niger was inoculated at the 1∶1 inoculum ratio. The βglucosidase activity and filter paper activity of the mixed fungi culture were 0.606 μmol/(min·mL) and 1.163 μmol/(min·mL), respectively, corresponding to a ratio of 0.521, which was higher than that of T. reesei under single cultivation. The yield of enzymatic hydrolysis was 65.61 % by the cocultivated cellulase, which was higher than those of singlecultivated cellulase and commercial cellulase. These results demonstrated that cocultivation of T. reesei and A. niger was suitable for optimization of cellulase components.

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Last Update: 2011-04-13