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二次回归正交设计在楸树离体生根培养中的应用(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2011年04期
Page:
47-50
Column:
研究论文
publishdate:
2011-07-31

Article Info:/Info

Title:
Application of quadratic regressive factorial experiment to in vitro rooting culture of Catalpa bungei
Author(s):
YU Yongming1WANG Junhui2*ZHANG Songzhi1MA Jianwei1LI Pingying1
1.Xiaolongshan Research Institute of Forestry, Tianshui 741022, China;2.Chinese Academy of Forestry,Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration,Beijing 100091, China
Keywords:
Catalpa bungei rooting rate quadratic regressive factorial experimentvariance analysis
Classification number :
S718
DOI:
10.3969/j.jssn.1000-2006.2011.04.010
Document Code:
A
Abstract:
Quadratic regressive factorial experiment and SPSS software was used to analyse and optimize concentrations of IBA and NAA added in rooting medium for the rooting rate,root length,root number,sprouting number,bud length,leaf number and shoot fresh weight of Catalpa bungei, we obtained a serial of regression equations. Using such equations to search for the best dosage of IBA and NAA to induce root in vitro culture of C. bungei. The results showed that, a certain concentration of NAA could promote the root differentiation and growth, and increase leaves, but there is no significant role to the growth of buds and sprouting number.The best dosage range of IBA is in 0.200—0.344 mg/L, and dosage range of NAA is in 0.023—0.073 mg/L, the rooting rate was achieved at 95 %, the probability of the event was 95 %.

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Last Update: 2011-08-13