我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|Table of Contents|

抗木材变色高效菌株B26-10的诱变选育(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2011年05期
Page:
51-54
Column:
研究论文
publishdate:
2011-09-30

Article Info:/Info

Title:
Screening and mutagenesis of Bacillus subtitles B2610 with high antifungal activity against wood sapstain
Author(s):
SUN WeiCHANG Jianmin*
College of Materials Science and Technology,Beijing Forestry University,Beijing 100083,China
Keywords:
antifungal activity against wood sapstain UV mutagenesis Bacillus subtilis B26 mutant B2610
Classification number :
S782.33
DOI:
10.3969/j.jssn.1000-2006.2011.05.011
Document Code:
A
Abstract:
Bacillus subtilis B26 with antifungal activity against wood sapstain was isolated from the soil samples of Betula platyphylla Suk. Cells of Bacillus subtilis B26, as an original strain were mutated by UV irradiation to improve its antifungal activity. The mutant B2610 was obtained due to enhanced activity in inhibiting Trichoderna viride 7258, Ceratocystis fimbriata 8430, and Alternaria alternata 5173. Compared to the performance of strain B26, the antifungal activity of mutant B2610 was promoted to 60 % for T. viride 7258, 46 % for C. fimbriata 8430, 25 % for A. alternata 5173, respectively. Successive subculturing of B2610 for 10 generations did not cause its antifungal activity loss. The antifungal activity of the supernatant, fermentation broth and the broth after treated with heating were also tested. The antifungal activity of fermentation broth was slightly higher than those of the supernatant; the fermentation broth lost antifungal activity after heating. The results indicated that the antifungal activity seems to be related with the production of active metabolites,and the active metabolites could be proteins. The successful control of sapstain can be obtained by the pretreatment of Bacillus subtilis B2610 before the attack of sapstain. The results indicated that Bacillus subtilis B2610 could be a potent organism for biocontrol agent in the field of wood protection.

References

[1]Morrell J J,Velicheti R K. Effect of Pseudomonas cepacia on the activity of a mixture of wood staining fungi on ponderosa pine sapwood[R]. Sweden: International Research Group on Wood Preservation, 1995.
[2]Feio S S, Barbosa A, Cabrita M, et al. Antifungal activity of Bacillus subtilis 355 against woodsurface contaminant fungi [J]. Journal of Industrial Microbiology & Biotechnology, 2004, 31(5): 199-203.
[3]Caldeira Ana T, Feio S S, Santos J M, et al. Bacillus amyloliquefaciens CCMI 1051 in vitro activity against wood contaminant fungi [J]. Annals of Microbiology, 2007, 57(1): 29-33.
[4]Croan S C, Highley T L. Antifungal activity in metabolites from Streptomyces rimosus [R]. Sweden: International Research Group on Wood Preservation, 1991.
[5]Bruce A, Highley T L. Control of growth of wood decay Basidiomycetes by Trichoderma spp. and other potentially antagonistic fungi [J]. Forest Products Journal, 1991, 41(2): 63-67.
[6]Srinivasan U, Highley T L, Croan S C, et al. Antagonistic effect of Trichoderma spp. against basidiospores[R]. Sweden: International Research Group on Wood Preservation, 1993.
[7]Seifert K A, Breuil C, Rossignol L, et al. Screening for microorganisms with the potential for biological control of sapstain on unseasoned lumber [J]. Material und Organismen, 1988, 23(2): 81-95.
[8]Cease K R, Blanchette R A, Highley T L. Interactions between Scytalidium species and brownor whiterot basidiomycetes in birch wood[J]. Wood Science and Technology, 1989, 23(2):151-161.
[9]李小清.白桦木材生物变色机理及防治研究[D].北京:北京林业大学,2008.
[10]Hiraki J, Hatakeyama M, Morita H, et al. Improved εpolyLlysine production of an S(2aminoethyl)Lcysteine resistant mutant of Streptomyces alblus [J]. Seibutsu Kogaku Kaishi, 1998, 76: 487-493.
[11]Podile A R, Prakash A P. Lysis and biological control of Aspergillus niger by Bacillus subtilis AF 1[J].Canadian Journal of Microbiology,1996, 42(4): 533-538.
[12]Bernier Jr R, Desrochers M, Jurasek L. Antagonistic effects between Bacillus subtilis and wood staining fungi [J]. Journal of the Institute of Wood Science, 1986, 10 (5): 214-216.
[13]Leifert C, Li H, Chidburee S, et al. Antibiotic production and biocontrol activity by Bacillus subtilis CL27 and Bacillus pumilus CL45 [J]. Journal of Applied Microbiology, 1995, 78(2): 97-108.
[14]Lee J K, Oh E S. Potentials for Biological Control of Blue Stain on Woods Caused by Ophiostomatoid Fungi [J]. The Plant Pathology Journal, 2000, 16(4):200-205.
[15]Cho B J, Kim N K, Cho N S, et al. Biocontrol of Blue Stain in Pine Wood with Lyophilized Mycelium of Ophiostoma quercus Albino Strain [J]. The Plant Pathology Journal, 2008, 24(3):309-316.

Last Update: 2011-09-30