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油茶花药离体培养影响因子研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2011年05期
Page:
125-128
Column:
油茶高效栽培研究专栏
publishdate:
2011-09-30

Article Info:/Info

Title:
Study of impact factor on the anther culture in vitro of Camellia oleifera
Author(s):
FAN XiaomingYUAN Deyi*
The Key Laboratory of Nonwood Forest Products of Forestry Ministry,Central South University of Forestry and Technology, Changsha 410004, China
Keywords:
Camellia yuhsiensis anther culture frequency of callus induction callus multiplication
Classification number :
S722;Q813
DOI:
10.3969/j.jssn.1000-2006.2011.05.028
Document Code:
A
Abstract:
The anther of Camellia yuhsiensis were selected as material. The effects of different culture conditions, including different developing period of pollen, time of low temperature pretreatment, and kinds of additives, on inducing callus were investigated. Then we further studied the influence of different plant growth regulators and ratio on callus proliferation. As a result, the best developing stage of pollen on inducing callus from oiltea anther is tetrad stage; the time of low temperature pretreatment should be below 10 days; and the best medium additives is AgNO3, with the high induction rate of 8102 %. In the culture of callus proliferation, the best combination is 6BA and NAA, and the best recipe of callus proliferation culture medium is MS+6BA(20 mg/L)+NAA(005 mg/L).

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Last Update: 2011-09-30