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松材线虫低温冷冻保存研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2011年06期
Page:
63-66
Column:
研究论文
publishdate:
2011-11-28

Article Info:/Info

Title:
Cryopreservation of the pine wood nematode(Bursaphelenchus xylophilus)
Author(s):
ZHU Lihua JI Jinyi ZHANG Wenli YE Jianren*
Jiangsu Key Laboratory for Prevention and Management of Invasive Species, College of Forest Resources and Environment,Nanjing Forestry University,Nanjing 210037,China
Keywords:
Bursaphelenchus xylophiluscryopreservationglycerinDMSO
Classification number :
S763
DOI:
10.3969/j.jssn.1000-2006.2011.06.013
Document Code:
A
Abstract:
The cryopreservation of Bursaphelenchus xylophilus was investigated. The effects of different cryoprotectants (1 %-5 % DMSO, 5 %-25 % glycerol) on cryopreservation of nematodes and the fertility and infectivity of cryonematodes were evaluated. The results indicated that the survival rate of B. xylophilus was up to (39.4±6.6) % or (375±21.8) % after storage for 1 day at -80 ℃ by using 15 % glycerin or 4 % DMSO respectively as cryoprotectant. And it didnt decline significantly after storage for 10 days or a month. When cryopreserved nematodes were plated onto Botrytis cinerea, they reproduced rapidly. Cryopreserved nematodes were as pathogenic as untreated nematodes to Japanese black pines.

References

[1]Mota M M, Braasch H, Bravo M A, et al. First report of Bursaphelenchus xylophilus in Portugal and in Europe[J]. Nematology, 1999(1): 727-734.
[2]Wang X D, Grewal P S. Rapid genetic deterioration of environmental tolerance and reproductive potential of an entomopathogenic nematode during laboratory maintenance[J]. Biological Control,2002,23(1):71-78.
[3]高景斌,户田忠雄.多次继代培养的松材线虫分离种致病力变异的研究[J].安徽农业科学,2006,34(13):3106-3107.
[4]Brenner S. The genetics of Caenorhabditis elegans [J]. Genetics,1974,77(1): 71-94
[5]Stiernagle T. Maintenance of C. elegans[G]//The C. elegans Research Community.WormBook. doi/10.1895/wormbook.1.101.1, http://www.wormbook.org. 
[6]Bridge J, Ham P J. A technique for the cryopreservation of viable juveniles of Meloidogyne graminicola [J]. Nematologica,1985,31(2):185-189.
[7]JacksonGegan M, James E R. Cryopreservation of Trichinella spiralis muscle stage larvae[J]. The American Journal of Tropical Medicine and Hygiene,1988,38:558-567.
[8]Triantaphyllou A C, Mccabe E. Efficient preservation of rootknot and cyst nematodes in liquidnitrogen[J]. Journal of Nematology,1989,21(3):423-426.
[9]Riga E, Webster J M. Cryopreservation of the pine wood nematode, Bursaphelenchus spp.[J]. Journal of Nematology, 1991,23: 438-440.
[10]Ogura N. A method for the cryopreservation of the pinewood nematode, Bursaphelenchus xylophilus[J]. Japanese Journal of Nematology, 2003,33(2): 77-79.
[11]Irdani T, Carletti B, Ambrogioni L, et al. Rapidcooling and storage of plant nematodes at 140 ℃[J]. Cryobiology,2006,52(3): 319-322.
[12]Elsen A, Vallterra S F, Van Wauwe T, et al. Cryopreservation of Radopholus similis, a tropical plantparasitic nematode[J]. Cryobiology, 2007,55(2): 148-157.
[13]黄任娥.感染松材线虫病松树滑刃目线虫研究[D].南京:南京林业大学,2007.
[14]朱丽华.松材线虫近交系建立及其低温冷冻保存研究[D].南京:南京林业大学,2010.
[15]James E R. Parasite cryopreservation by vitrification[J]. Cryobiology, 2004, 49(3): 201-10.
[16]Galway N J, Curran J. Cryopreservation of Pratylenchus spp.[J]. Journal of Nematology,1995,27:483-485.
[17]Vanderbeek H J G, Veldhuis W B J, Zijlstra C, et al. Preservation of Meloidogyne hapla and M. chitwoodi in liquid nitrogen: differences in response between populations[J]. Fundamental and Applied Nematology,1996,19(3):227-234.

Last Update: 2011-11-28