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3种杀虫剂对杨树羧酸酯酶的抑制效果(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2012年02期
Page:
81-85
Column:
研究论文
publishdate:
2012-04-10

Article Info:/Info

Title:
Inhibition effect of three insecticides on carboxylesterase from poplar
Author(s):
TANG FangZHU Yingjia
Jiangsu Key Laboratory for Prevention and Management of Invasive Species, College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037, China
Keywords:
poplar omethoate imidacloprid fipronil carboxylesterase activity
Classification number :
S767
DOI:
10.3969/j.jssn.1000-2006.2012.02.017
Document Code:
A
Abstract:
The optimum reaction condition of carboxylesterase in poplar was studied using the biochemistry methods. The results indicated that the optimum enzyme dilution factor of carboxylesterase was 10, and the optimum substrate concentration was 3×10-4 mol/L, the optimum pH value was 7.0, and the optimum reaction time was 15 min, and the optimum temperature 30 ℃, for carboxylesterase in poplar. Furthermore, the effect on carboxylesterase from poplar by omethoate, imidacloprid and fipronil was determined. The results showed that carboxylesterase activity of poplar was inhibited by 400,1 600,3 200 and 6 400 mg/mL omethoate, and the inhibition effect of 6 400 mg/mL omethoate was strongest. The carboxylesterase activity of poplar didn’t change, after being treated by 50,100,200,400 and 800 mg/mL imidacloprid for 48 h, and the activity decreased by 400 and 800 mg/mL imidacloprid for 96 h. The carboxylesterase activity of poplar decreased after being treated by 400 mg/mL fipronil for 48 h, and by 800 mg/mL fipronil for 96 h.

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Last Update: 2012-04-10