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硫酸二甲酯对毛泡桐丛枝病幼苗植原体及SSR扩增位点的影响(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2012年03期
Page:
5-8
Column:
研究论文
publishdate:
2012-05-30

Article Info:/Info

Title:
Effect of dimethyl sulphate on phytoplasma of Paulownia tomentosa seedling infected by witches’ broom and its DNA loci at SSR level
Author(s):
FAN Guoqiang ZHAO Gaili ZHAI Xiaoqiao CAO Xibing
Institute of Paulownia, Henan Agricultural University, Zhengzhou 450002, China
Keywords:
Paulownia tomentosa witches’ broom dimethyl sulphate(DMS) morphological change SSR
Classification number :
S763
DOI:
10.3969/j.jssn.1000-2006.2012.03.002
Document Code:
A
Abstract:
The effect of different concentration of dimethyl sulphate(DMS)on the morphological and phytoplasma changes of Paulownia tomentosa seedlings infected by witches’ broom and DNA base sequences changes at SSR level were investigated by morphological observation, net-PCR and SSR analysis. The results indicated that the seedlings infected by witches’ broom which were treated with optimal concentration DMS might be cured, however there were still exist phytoplasma after treated with 15 mg/L DMS for 5 h. The seedlings were not alive when DMS concentration was larger than 150 mg/L. Moreover, the DNA loci at SSR level of the healthy, infected and the infected-treated seedlings were the same.

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Last Update: 2012-05-30