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慈竹CCoAOMT基因的克隆及生物信息学分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2012年03期
Page:
17-22
Column:
研究论文
publishdate:
2012-05-30

Article Info:/Info

Title:
Cloning of CCoAOMT gene in Neosinocalamus affinis and its bioinformatics analysis
Author(s):
WU XiaoyuHU Shanglian* CAO YingLU Xueqin REN Peng ZHOU Meijuan LI Xiaorui
College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010,China
Keywords:
Neosinocalamus affinis caffeoyl-coA O-methyltransferase gene clone bioinformatics analysis
Classification number :
S718.46; Q785
DOI:
10.3969/j.jssn.1000-2006.2012.03.005
Document Code:
A
Abstract:
Caffeoyl-coA O-methyltransferase(CCoAOMT)is a key enzyme in lignin biosynthesis. A gene of CCoAOMT family with the cDNA length of 1 080 bp was cloned by RT-PCR and RACE method from young stems of Neosinocalamus affinis and bioinformatics analyzed. The full-length sequence of the CCoAOMT gene from N. affinis was successfully cloned in this studies. The results showed that the gene contained an open reading frame of 777 bp, which encoded a polypeptide of 258 amino acids, the molecular weight of the predicted protein was 28.861 ku, its theoretical PI was 5.33, the gene’s secondary structure consist of α-helix, β-turn, extended strand and random coil; the proportions were 42.25%,8.91%, 32.95% and 15.89%, respectively. The BLASTp results indicated that N. affinis had the highest identity with Bambusa oldhamii. There is a base deletion at the tenth site, and has five variable sites compared with CCoAOMT of B. oldhamii. One relative conserved disordered domain was found in the CCoAOMT gene from N. affinis, B. oldhamii, Phyllostachys edulis and Zea mays. The expression of NaCCoAOMT1 showed that the gene highly expressed in young leaves, upper and middle parts of shoots and stem. This is the first time that NaCCoAOMT1 was cloned in N. affinis. The sequence of NaCCoAOMT1 has been submitted to GenBank with the accession number JF742462.

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Last Update: 2012-05-30