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利用ISSR和SRAP标记分析油茶遗传多样性(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2012年05期
Page:
19-25
Column:
研究论文
publishdate:
2012-09-30

Article Info:/Info

Title:
Genetic diversity of Camellia oleifera using ISSR and SRAP markers
Author(s):
PENG Fangren1WU Yingying1 HAO Mingzhuo1CHEN Longsheng2 CHEN Yongzhong2
1.College of Forest Resource and Environment, Nanjing Forestry University, Nanjing 210037, China; 
2. Forestry Science Academy of Hunan Prefecture, Changsha 410004, China
Keywords:
Camellia oleifera genetic diversity ISSR SRAP
Classification number :
S722
DOI:
10.3969/j.jssn.1000-2006.2012.05.003
Document Code:
A
Abstract:
In this paper, two kinds of molecular markers, ISSR and SRAP were used to assess the genetic diversity in 192 accessions of Camellia oleifera. Ten primers were screened from 100 primers of ISSR, 118 bands were amplified, among which 104 bands were polymorphic bands, the percentage of polymorphic band was 8814 %, the Neis genetic similarity coefficients ranged from 052 to 100. Twelve pairs primers of SRAP were selected out, 284 bands were amplified, of which 244 bands (85.37 %) were polymorphic, and the Neis genetic similarity coefficients ranged from 065 to 095. The correlation of the genetic similarity matrix among the ISSR, SRAP and the combination data were higher. It indicated that the two methods had highly consistent in the genetic diversity analysis of C. oleifera, furthermore the SRAP technique was more reliable. According to the UPGMA method, we made the cluster analysis by using the NTSYS software. The 192 germplasms of C. oleifera could be divided into 6 groups at the similarity coefficient of 068 by ISSR marker; they also could be divided into 6 groups at the similarity coefficient of 070 by SRAP marker. This indicated that the C. oleifera germplasms had close relationship. However, the dendrograms cluster analysis showed that some germplasms probably represent different genetic background and high genetic diversity.

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Last Update: 2012-09-30