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橡胶树胶乳C-乳清死皮相关蛋白的鉴定及分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2012年05期
Page:
37-41
Column:
研究论文
publishdate:
2012-09-30

Article Info:/Info

Title:
Identification and analysis of proteins in the latex Cserum related to the tapping panel dryness in Hevea brasiliensis
Author(s):
ZHOU Xuemei12 YANG Lifu1 WANG Zhenhui1 ZOU Zhi1 YUAN Kun1*
1. Rubber Research Institute, Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture, Danzhou 571737, China;
2. College of Agriculture, Hainan University, Haikou 570100, China
Keywords:
tapping panel dryness (TPD)latex Cserumidentification of protein twodimensional gel electrophoresis (2DE protein)mass spectrometry(MALDITOF MS)
Classification number :
S794.1;Q503
DOI:
10.3969/j.jssn.1000-2006.2012.05.006
Document Code:
A
Abstract:
The tapping panel dryness (TPD) in Hevea brasiliensis is a significant limit factor influencing natural rubber production. In this experiment, the twodimensional gel electrophoresis (2DE) was used to study the differentiation of protein expression profiles of latex Cserum between TPD trees and healthy ones of H. brasiliensis. Thirtyone differentially expressed protein spots were obtained by software analysis. After these spots were subjected to matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDITOF MS) followed by searching for NCBInr database, ten spots were successfully identified. Six of them were identified as the unknown proteins and the remaining 4 were the chain A, structure of ubiquitinlike protein, Rub1; β1, 3glucanase; AIR9 protein and flagellar inner dynein arm heavy chain 11. These proteins might play important roles in the process of TPD occuring.

References

[1]刘志昕,郑学勤.橡胶树死皮病的发生机理和假说[J].生命科学研究,2002,6(1):83-85.
[2]覃宝祥,胡新文,邓晓东,等.橡胶树死皮病的形成及其机制[J].植物生理学通讯,2005,41(6):827-830.
[3]Suvachittanont W, Wititsuwannakul R. 3Hydroxy3methylglutarylcoenzyme a synthase in Hevea brasiliensis [J]. Phytochemistry,1995,40(3): 757-761.
[4]Posch A, Chen Z, Wheele C, et al. Characterization and identification of latex allergens by twodimensional electrophoresis and protein microsequencing [J]. J Allergy Clin Immunol, 1997,99(3): 385-395.
[5]Lacrotte R, Vichitcholchai N, Chrestin H, et al. Protein markers linked to the Tapping Panel Dryness (TPD) of Hevea brasiliensis[C]//. IRRDB Workshop on Tapping Panel Dryness in Hevea brasiliensis. Haikou: CATAS, 1997.
[6]Sookmark U, PujodeRenaud V, Chrestin H, et a1.Characterization of polypeptides accumulated in the latex cytosol of rubber tree affected by the tapping panel dryness syndrome[J].Plant Cell Physiol,2002,43:1323-1333.
[7]Blum H, Beier H, Gross H. Improved silver staining of plant proteins,RNA and DNA in polyacrylamide gels [J]. Electrophoresis, 1987, 17 (8): 93-99.
[8]Fernandez J, Gharahdaqhi F, Mische S M. Routine identification of proteins from sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDSPAGE)gel or polyvinyl difluoride membranes using matrix assisted laser desorption/ionizationtime of flightmass spectrometry (MALDITOFMS) [J]. Electrophoresis, 1998, 19(6): 1036-1045.
[9]Gharahdaghi F,Weinberg C R,Meagher D A, et al. Mass spectrometric identification of proteins from silverstained polyacrylamide gel:a method for the removal of silverions to enhance sensitivity[J]. Electrophoresis, 1999,20 (3):601-605.
[10]袁坤,王真辉,丁璇,等.巴西橡胶树树皮蛋白质组学分析体系的构建[J].南京林业大学学报:自然科学版,2009, 33(6): 146-149.
[11]Vierstra R D,Callis J. Polypeptide tags, ubiquitous modifiers for plant protein regulation [J]. Plant Mol Biol, 1999, 41: 435-442.
[12]Melchior F. SUMOnonclassical ubiquitin [J]. Annu Rev Cell Dev Biol, 2000, 16: 591-626.
[13]Kurepa J, Walker J M, Smalle J,et al. The small ubiquitinlike modifier (SUMO) protein modification system in Arabidopsis. Accumulation of SUMO1 and 2 conjugates is increased by stress [J]. Biol Chem, 2003, 278: 6862-6872.
[14]Li D, Deng Z, Chen C, et al. Identification and characterization of genes associated with tapping panel dryness from Hevea brasiliensis latex using suppression subtractive hybridization [J]. BMC Plant Biol, 2010, 10(1): 140-152.
[15]Venkatachalam P, Thulaseedharan A, Raghothama K. Identification of expression profiles of tapping panel dryness (TPD) associated genes from the latex of rubber tree (Hevea brasiliensis Muell. Arg.) [J]. Planta, 2007, 226: 499-515.
[16]Anuratha C S, Zen K C, Cole K C, et al. Induction of chitinases and β1, 3glucanases in Rhizoctonia solani infected rice plants: Isolation of an infectionrelated chitinase cDNA clone[J]. Physiol Plant, 1996, 97: 39-46.
[17]Mauch F, Hadwiger L A, Boller T. Antifungal hydrolases in pea tissue: I. Purification and characterization of two chitinases and two β1, 3glucanases differentially regulated during development and in response to fungal infection[J]. Plant Physiol, 1988, 87: 325-333.
[18]Mauch F, MauchMani B, Boller T. Antifungal hydrolases in pea tissue: II. Inhibition of fungal growth by combinations of chitinase and β1, 3 glucanase[J]. Plant Physiol, 1988, 88: 936-942.
[19]SanchezBallesta M T, Zacarias L,Granell A, et al. β1,3Glucanase gene expression as a molecular marker for postharvest physiological disorders in citrus fruit and its hormonal regulation[J]. Plsharvest Bio Tec, 2008, 48(1): 146-149.
[20]Wan L, Zha W, Cheng X, et al. A rice β1, 3glucanase gene Osg1 is required for callose degradation in pollen development [J]. Planta, 2011, 233 (2): 309-323.
[21]闫洁,陈守才,夏志辉. 橡胶树死皮病胶乳C乳清差异表达蛋白质的筛选与鉴定[J].中国生物工程杂志, 2008, 28(6): 28-36.

Last Update: 2012-09-30