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青钱柳幼胚愈伤组织的诱导(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2012年05期
Page:
47-50
Column:
研究论文
publishdate:
2012-09-30

Article Info:/Info

Title:
Callus induction from young embryos of Cyclocarya paliurus
Author(s):
ZHANG JinfengFANG Shengzuo*SHANG XulanXU Xizeng
College of Forest Resources and Environment, Nanjing Forestry University,Nanjing 210037,China
Keywords:
Cyclocarya paliurus young embryo callus media plant growth regulator
Classification number :
S722;Q944.5
DOI:
10.3969/j.jssn.1000-2006.2012.05.008
Document Code:
A
Abstract:
Using young embryos of Cyclocarya paliurus as explants, we investigated the effects of embryo development period, basic media, types and the concentrations of plant growth regulators on callus induction of C. paliurus. The results showed that there were obvious differences in the shape of callus among different embryo development periods, and the middle cotyledon embryos were most suitable for callus induction. As a basic media, improved DKW medium was the best selection for young embryo callus induction, of which induction rate could hit to 88.89 % with no browning phenomenon. The medium containing 2,4-D and KT was more appropriate for callus induction. The 2,4-D (0.5 mg/L)+KT (2.0 mg/L) as well as 2,4-D (1.0 mg/L)+KT (1.0 mg/L) were optimal for callus induction of C. paliurus young embryo, and the induction rates were 80.00 % and 78.33 % respectively.

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Last Update: 2012-09-30