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杂种鹅掌楸体胚苗根尖染色体核型分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2012年06期
Page:
13-16
Column:
研究论文
publishdate:
2012-12-05

Article Info:/Info

Title:
Karyotype analysis of the Liriodendron chinense×L. tulipifera root tip chromosome
Author(s):
LIU Guangxin CHEN Jinhui SUN Jie YU Tianchi SHI Jisen*
Key Laboratory of Forest Genetics and Biotechnology Ministry of Education, Nanjing Forestry University, Nanjing 210037, China
Keywords:
Liriodendron chinense×L.tulipifera chromosomekaryotype analysis
Classification number :
Q943
DOI:
10.3969/j.jssn.1000-2006.2012.06.003
Document Code:
A
Abstract:
Icewater mixture,07 mmol/L cycloheximide and 02 % colchicine were used as pretreated reagent for Liriodendron hybrids root tips of testtube plantlet by different time grads. Then the root tips were squashed with Carbol fuchsin and observed with microscope. It had been proved that all these three pretreatments of icewater mixture for 6 h, 07 mmol/L cycloheximide for 2 h, and 02 % colchicine for 4 h could obtain metaphase chromosome specimen, but by comparison, root tips pretreated with cycloheximide and colchicine could give much better chromosome morphology. Then Karyotype of the Liriodendron chinense×L.tulipifera was studied with chromosome samples prepared from root tip by 07 mmol/L cycloheximide for 2 h. The result showed that there were 38 chromosome in Liriodendron chinense×L.tulipifera and its karyotype formula was 2n=38=26m+8sm+2st+2t and the karyotypes of Liriodendron chinense×L.tulipifera belong to category 2B of Stebbins.

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Last Update: 2012-11-30