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|Table of Contents|

云芝菌株胞外多糖的制备及纯化(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2012年06期
Page:
85-89
Column:
研究论文
publishdate:
2012-12-05

Article Info:/Info

Title:
Preparation and purification of extracellular polysaccharide from Coriolus versicolor
Author(s):
SUN JingYOU YiJIANG FaxianYANG RuiCAI JunliZHAO Linguo*
Jiangsu Key Lab of Biomass Based Green Fuels and Chemicals, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
Keywords:
Coriolus versicolor extracellular polysaccharide response surface methodology purification
Classification number :
S567;Q943
DOI:
10.3969/j.jssn.1000-2006.2012.06.017
Document Code:
A
Abstract:
To study the optimization of culture conditions on the extracellular polysaccharides Coriolus versicolor, the response surface methodology (RSM) based on the single factor tests were applied. According to regression analysis, the optimal culture conditions were culture time 24 days, broth content 676 mL, and initial pH 51. In order to get the purified polysaccharide, alcohol precipitation and DEAE Sepharose CL6B purification were used. The polysaccharide was identified the uniform component by PAGE. The HPLC results showed that the components of monosaccharide contain at least glucose and xylose, without peptides.

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Last Update: 2012-11-30