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耐热α-葡萄糖苷酶的诱导条件及重组酶的固定化(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2012年06期
Page:
90-94
Column:
研究论文
publishdate:
2012-12-05

Article Info:/Info

Title:
Inducement condition and immobilization of thermostable αglucosidase produced by Escherichia coli
Author(s):
PEI Jianjun L Xiangxiang XIE Jingcong ZHAO Linguo*
Jiangsu Key Lab of Biomass Based Green Fuels and Chemicals, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
Keywords:
thermostable αglucosidase inducement condition immobilization of enzyme
Classification number :
TQ925
DOI:
10.3969/j.jssn.1000-2006.2012.06.018
Document Code:
A
Abstract:
Several factors influencing αglucosidase production with the recombinant Escherichia coli haroring the JM109/pBV220GLZ were investigated. The results showed that the best inducement conditions were as follows: induction temperature 40 ℃, induction time 14 h, and at OD600 of 2.0. To determine the optimum conditions of immobilization, the concentration of sodium alginate, CaCl2, and glutaraldehyde, and solidifying time were studied. The results showed that the optimum operations were: 20 g/L sodium alginate, 20 g/L CaCl2, 1.0 % glutaraldehyde, solidifying time 2 h. Under the optimum condition, the recovery rate reached 81 %. After 15 times repeated operations, the enzyme activity remained 75 %, showing good operational stability. Thus, the superior stability of the recombinant enzyme laid the base for largescale application.

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Last Update: 2012-11-30