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培养基组成对里氏木霉合成β-甘露聚糖酶的影响(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2013年01期
Page:
101-104
Column:
研究论文
publishdate:
2013-01-15

Article Info:/Info

Title:
Effects of medium components on β-mannanase production by Trichoderma reesei
Author(s):
WANG Jing LI Xin ZHU Junjun YU Shiyuan YONG Qiang*
College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China
Keywords:
β-mannanase Trichoderma reesei medium optimization
Classification number :
Q814
DOI:
10.3969/j.issn.1000-2006.2013.01.018
Document Code:
A
Abstract:
In order to improve the synthesis of β-mannanase by Trichoderma reesei,and determinate enzyme activity,fermentation medium of nutritional composition and proportion were investigated,which included carbon sources, carbon source concentration, nitrogen sources and ratio, carbon to nitrogen(mC:mN)ratio of four main factors on the biosynthesis of β-mannanase by Trichoderma reesei.The results showed that 20 g/L microcrystalline cellulose used as the carbon source, ammonium sulfate-urea mixture with the ratio of 1:1 used as the nitrogen source, the mC:mN=4 of culture medium composition were optimal nutrient for β-mannosidase synthesis of Trichoderma reesei.The highest β-mannanase activity and β-mannosidase activity were 4.48 and 0.04 μmol/(min·mL), respectively.The maximal β-mannanase activity was gained after 96 h fermentation.

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Last Update: 2013-01-15