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|Table of Contents|

柽柳ThPR1基因的克隆与表达分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2013年02期
Page:
45-49
Column:
研究论文
publishdate:
2013-03-31

Article Info:/Info

Title:
Clone and expression analysis of ThPR1 gene in Tamarix hispida
Author(s):
ZHANG KaiminWANG Yucheng YANG Guiyan GAO Caiqiu*
State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin 150040, China
Keywords:
Tamarix hispida pathogenesis-related protein qRT-PCR NaCl stress PEG stress ABA treatments
Classification number :
Q943.2
DOI:
10.3969/j.issn.1000-2006.2013.02.008
Document Code:
A
Abstract:
Pathogenesis-related proteins(PRs)in plants are proteins in plants that induced by biological or abiotic stresses. In this study, the full length cDNA of a PR1(named ThPR1)gene was isolated from seven transcriptome cDNA librarys of T. hispida under NaHCO3 stress. The ThPR1 cDNA was 756 bp, and open reading frame(ORF)was 537 bp, encoding a protein of 178 amino acid residues with the molecular mass of 20.53 ku and theoretical pI of 9.75. The expressions of ThPR1 in T. hispida responding to NaCl, PEG and ABA were further investigated using quantitative RT-PCR. The results showed that ThPR1 was induced in roots and leaves of T.hispida under NaCl stress, but inhibited under PEG and ABA treatments.

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Last Update: 2013-03-31