我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|Table of Contents|

白桦SPL8转录因子基因的分离及转录表达分析(PDF/HTML)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2013年03期
Page:
17-22
Column:
研究论文
publishdate:
2013-05-20

Article Info:/Info

Title:
Isolation and transcription expression analysis of SPL8 transcription factors gene of Betula platyphylla
Author(s):
GUAN Minxiao LIU Xuemei* ZHANG Yan LIU Ying SUN Fengbin
College of Life Science,Northeastern Forest University, Harbin 150040, China
Keywords:
Betula platyphylla SPL8 gene transcript qRT-PCR mutant flower development differential expression
Classification number :
Q943.2
DOI:
10.3969/j.issn.1000-2006.2013.03.004
Document Code:
-
Abstract:
A 1 230 bp full-length cDNA of SPL8 gene was isolated from Betula platyphylla Suk. using RACE(rapid amplification of cDNA ends)and RT-PCR(reverse transcription polymerase chain reaction). SPL8 gene contains an open reading frame of 930 bp which encodes 309 amino acid with the deduced molecular mass around 34.32 ku and the theoretical isoelectric point of 9.08.The gene was named BplSPL8 and submitted to GenBank with accession number JQ354964.The homology of amino acid compared with Arabidopsis thaliana AtSPL8, Glycine max GmSPL8, Solanum chacoense ScSPL8 and Vitis vinifera VvSPL8 was 55.4%,73.1%,63.9% and 70.8%, respectively. The qRT-PCR result showed that BplSPL8 is expressed in all different tissues, the expression was the highest in the male inflorescences, whereas lowest in the seed and pollen. The expression level changed at different development stages in the male inflorescences. In addition, the expression levels of BplSPL8 in mutant was below that in wild-type in all different tissues, which suggests that BplSPL8 may play an important role on the development’s specific stage of the male inflorescences in the Betula and its abnormal expression may be associated with male sterility.

References

[1] Klein J, Saedler H, Huijser P. A new family of DNA binding proteins includes putative transcriptional regulators of the Antirrhinum majus floral meristem identity gene SQUAMOSA[J]. Mol Gen Genet, 1996, 250:7-16.
[2] Cardon G H, Hohmann S, Nettesheim K, et al. Functional analysis of the Arabidopsis thaliana SBP-box gene SPL3:a novel gene involved in the floral transition[J]. Plant J, 1997, 12:367-377.
[3] Lannenpaa M, Janonen I, Holtta-Vuori M, et al. A new SBP-box gene BpSPL1 in silver birch(Betula pendula)[J]. Plant Physiol,2004, 120:491-500.
[4] Becraft P W, Bongard-Pierce D K, Sylvester A W, et al. The LIGULELESS-1 gene acts tissue specifically in maize leaf development[J]. Dev Biol, 1990,141:220-232.
[5] Moreno M A, Harper L C, Krueger R W, et al. Liguleless1 encodes a nuclear-localized protein required for induction of ligules and auricles during maize leaf organogenesis[J].Genes Dev, 1997,11:616-628.
[6] Manning K, Tor M, Poole M, et al. A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening [J]. Nat Genet, 2006,38:948-952.
[7] 赵晓初,李贺,代红艳,等.草莓 miR156 靶基因 SPL9 的克隆与表达分析[J].中国农业科学,2011,44(12):2515-2522. Zhao X C, Li H, Dai H Y, et al. Cloning and expression analysis of miR156-targeted SPL9 gene from strawberry[J]. Scientia Agricultura Sinica, 2011,44(12):2515-2522.
[8] Wang Y, Hu Z, Yang Y, et al. Function annotation of an SBP-box gene in Arabidopsis based on analysis of co-expression networks and promoters[J]. Int J Mol Sci, 2009,10:116-132.
[9] Wu G, Poethig R S. Temporal regulation of shoot development in Arabidopsis thaliana by miR156 and its target SPL3[J]. Development, 2006,133:3539-3547.
[10] Nan Y, Cai W J, Wang S C, et al. Temporal control of trichome distribution by microRNA156-targeted SPL genes in Arabidopsis thaliana[J]. Plant Cell, 2011, http://dx.doi.org/10.1105/tpc.109.072579.
[11] Usami T, Horiguchi G, Yano S, et al. The more and smaller cells mutants of Arabidopsis thaliana identify novel roles for SQUAMOSA PROMOTER BINDING PROTEIN-LIKE genes in the control of heteroblasty [J]. Development, 2009,136,955-964.
[12] Wang H, Nussbaum-Wagler T, Li B, et al. The origin of the naked grains of maize[J]. Nature, 2005,436:714-719.
[13] Jiao Y, Wang Y, Xue D, et al. Regulation of OsSPL14 by OsmiR156 defi nes ideal plant architecture in rice [J]. Nat Genet, 2010,42:541-544.
[14] Unte U S, Sorensen A M, Pesaresi P, et al. SPL8, an SBP-box gene that affects pollen sac development in Arabidopsis[J]. Plant Cell, 2003,15:1009-1019.
[15] Zhang Y, Schwarz S, Saedler H, et al. SPL8,a local regulator in a subset of gibberellin-mediated developmental processes in Arabidopsis[J]. Plant Mol Biol, 2007,63:429-439.
[16] Eriksson M, Moseley J L, Tottey S, et al. Genetic dissection of nutritional copper signaling in chlamydomonas distinguishes regulatory and target genes[J]. Genetics, 2004,168:795-807.
[17] Stone J, Liang X, Nekl E, et al. Arabidopsis AtSPL14,a plant-specic SBP-domain transcription factor, participates in plant development and sensitivity to fumonisin B1[J]. Plant J, 2005, 41:744-754.
[18] 刘雪梅,杨传平.白桦雌雄花发育周期的时序特征[J].林业科学,2006,42(12):28-32. Liu X M, Yang C P. Temporal characteristics of developmental cycles of female and male flowers in Betula platyphylla in northeastern China[J]. Scientia Silvae Sinicae, 2006, 42(12):28-32.
[19] 刘雪梅,周菲,邢磊,等.白桦雄花突变体及其细胞学特征[J].东北林业大学学报,2010,38(6):1-3,14. Liu X M, Zhou F, Xing L, et al. Cytological characteristics of male floral mutantin Betula platyphylla[J]. Jouranl of Northeast Forestry University, 2010,38(6):1-3,14.
[20] 施季森.迎接21世纪现代林木生物技术育种的挑战[J].南京林业大学学报,2000,24(1):1-6. Shi J S. To meet the 21st century modern forest biotechnology breeding challenge[J].Journal of Nanjing Forestry Unviersity, 2000,24(1):1-6.
[21] 张妍,刘瀛,孙丰宾,等.白桦APETALA2(AP2)转录因子基因的分离及其表达[J].林业科学研究,2012,25(2):254-260. Zhang Y, Liu Y, Sun F B, et al. Isolation and expression of APETALA2 transcription factor gene in Betula platyphylla[J]. Forest Research, 2012,25(2):254-260.
[22] 曾凡锁,南楠,詹亚光.富含多糖和次生代谢产物的白桦成熟叶中总RNA的提取[J].植物生理学通讯,2007,43(5):913-916. Zeng F S, Nan N, Zhan Y G. Extraction of total RNA from mature leaves rich in polysaccharides and secondary metabolites of Betula platyphylla Suk.[J].Plant Physiology Communications, 2007,43(5):913-916.
[23] 戴超,刘雪梅,周菲.白桦基因表达半定量RT-PCR中内参基因的选择[J].经济林研究,2011,29(1):34-39. Dai C, Liu X M, Zhou F. Selection of internal control genes in semi-quantitative RT-PCR in Betula platyphylla[J].Economic Forest Researches, 2011,29(1):34-39.
[24] Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T))[J]. Methods Methods, 2001,25:402-408.
[25] Yamasaki K, Kigawa T, Inoue M, et al. A novel zinc-binding motif revealed by solution structures of DNA-binding domains of Arabidopsis SBP-family transcription factors[J]. J Mol Biol, 2004,337(1):49-63.
[26] Birkenbihl R P, Jach G, Saedler H, et al. Functional dissection of the plant-specific SBP-domain, overlap of the DNA-binding and nuclear localization domains [J]. J Mol Biol, 2005,352:585-596.
[27] 吴晓宇,胡尚连,曹颖,等.慈竹 CCoAOMT 基因的克隆及生物信息学分析[J].南京林业大学学报:自然科学版,2012,36(3):17-22. Wu X Y, Hu S L, Cao Y, et al. Cloning of CCoAOMT gene in Neosinocalamus affinis and its bioinformatics analysis[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2012,36(3): 17-22.
[28] 张萍,潘惠新,黄敏仁,等.杨树抗杨四瘿螨相关基因的表达分析[J].南京林业大学学报:自然科学版, 2009, 33(4):8-12. Zhang P, Pan H X, Huang M R, et al. Differentially expressed gene analysis of populus induced by Tetra lobulifera(Keifer)[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2009, 33(4): 8-12.
[29] Yang J H, Fang Z M, Yu J Q. Relationship between cytoplasmic male sterility and SPL-like gene expression in stem mustard [J]. Physiol Plantarum, 2008,133:426-434.

Last Update: 2013-05-31