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湿加松胚性愈伤组织的程序降温技术研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2013年06期
Page:
1-5
Column:
研究论文
publishdate:
2013-11-27

Article Info:/Info

Title:
Cryopreservation of embryogenic callus of Pinus elliottii × Pinus caribaea using control freezing
Article ID:
1000-2006(2013)06-0001-05
Author(s):
LIU Weidong CHEN Jinhui ZHOU Yanwei ZHAO Yaqi SHI Jisen*
Key Laboratory of Forest Genetics and Biotechnology Ministry of Education,Nanjing Forestry University, Nanjing 210037, China
Keywords:
Pinus elliottii× Pinus caribaea embryogenic callus cryopreservation
Classification number :
S723.1+34
DOI:
10.3969/j.issn.1000-2006.2013.06.001
Document Code:
A
Abstract:
Cryopreservation of callus of Pinus elliottii × Pinus caribaea were conducted in this research. The results showed that the best process of cryopreservation for the embryogenic callus of P. elliottii × P. caribaea was as follows: the callus were sub-cultured for 9-12 days in callus proliferation medium firstly and were pre-cultured in 0.5 mol/L sorbol for 4 days, and then pretreated 20 minutes in buffer of 0.6 mol/L sorbol + 10% DMSO, cooled to -40 ℃ at rate of -1 ℃/min, maintained at -40 ℃ for 10 min, preserved in liquid nitrogen after cooled to -90 ℃ at rate of -5 ℃/min. The resuscitate callus were kept in 37 ℃ water bath, clean three times in 1 mol/L sorbol liquid medium, passage in solid medium support by filter paper. New embryogenic callus will be produced within one month.

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Last Update: 2013-11-30