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|Table of Contents|

柳杉SSR引物的筛选及反应体系的优化(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2013年06期
Page:
17-21
Column:
研究论文
publishdate:
2013-11-27

Article Info:/Info

Title:
Optimization of SSR-PCR system and SSR primer screening in Cryptomeria fortunei
Article ID:
1000-2006(2013)06-0017-05
Author(s):
XU Jin LIU Ziliang
Key Laboratory of Forest Genetics and Biotechnology Ministry of Education, Nanjing Forestry University, Nanjing 210037, China
Keywords:
Cryptomeria fortunei SSR primer primer screening optimization of SSR-PCR system
Classification number :
S722
DOI:
10.3969/j.issn.1000-2006.2013.06.004
Document Code:
A
Abstract:
A orthogonal design was used to optimize SSR amplification for Cryptomeria fortunei in five factors:Mg2+,DNA template,Taq DNA polymerase,dNTP, primer. The results were shown as follows:(1)A suitable 10 μL SSR reaction system contained Mg2+ 2.0 mmol/L, each of four dNTPs 0.15 mmol/L, primers 0.3 μmol/L, Taq polymerase 0.5 μmol/min, DNA template 20 ng.(2)Twenty-six SSR primers were screened from 106 primers existing in Cryptomeria,which had high polymorphism and stability.(3)The best annealing temperature of twenty-six SSR primers were optimized. The results provided references for genetic diversity research of C. fortunei using SSR marker.

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Last Update: 2013-11-30