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杂交竹梢枯病拮抗菌的筛选及抗菌蛋白分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2013年06期
Page:
27-32
Column:
研究论文
publishdate:
2013-11-27

Article Info:/Info

Title:
Selection of an antagonistic bacterium against Arthrinium phaeospermum and its antibacterial protein analysis
Article ID:
1000-2006(2013)06-0027-06
Author(s):
LI Shujiang LIANG Man ZHU Tianhui* YANG Changqing
College of Forestry, Sichuan Agricultural University, Ya’an 625014, China
Keywords:
Arthrinium phaeospermum antagonistic bacterial antibacterial protein physicochemical characteristics
Classification number :
S763.1
DOI:
10.3969/j.issn.1000-2006.2013.06.006
Document Code:
A
Abstract:
An antagonistic bacterial strain YB-15 against Arthrinium phaeospermum was isolated from Bambusa pervariabilis×Dendrocalamopsis grandis. The strain was identified as Bacillus velezensis by the morphological, physiological and biochemical characteristics, and 16SrDNA sequence analysis. One of the antibacterial protein was purified by using ammonium sulfate precipitation, the Sephadex G-50 chromatography, High Q Sepharose Fast Flow chromatography and Sephadex G-75 chromatography.The molecular weight of the antibacterial protein was estimated to be 31.0 ku by SDS-PAGE analysis.Stability test showed that the antibacterial protein was resistant to proteinase K and trypsin,thermal stable, sensitive to pH and ultraviolet, had different sensitivity to organic solvents.

References

[1] 朱天辉, 黄宗超, 高强章, 等.撑×绿杂交竹梢枯病病原及发生规律研究[J]. 中国森林病虫, 2009, 28(2):10-12,31. Zhu T H, Huang Z C, Gao Q Z, et al. Pathogen and occurrence regularity of Bambusa ervariabilis × Dendrocalamopsis daii blight[J]. Forest Pest and Disease, 2009, 28(2):10-12,31.
[2] 杨佐忠, 叶建仁. 杂交竹枯梢病的病原鉴定[J]. 四川农业大学学报, 2004, 22(3):225-227. Yang Z Z, Ye J R. Identification on pathogen of hybrid bamboo blight[J]. Journal of Sichuan Agricultural University, 2004, 22(3):225-227.
[3] 朱天辉, 黄宗超, 高强章, 等. 撑×绿杂交竹梢枯病调查及空间分布型研究[J]. 四川林业科技, 2009, 30(1):29-31,37. Zhu T H, Huang Z C, Gao Q Z, et al. Research on survey and space distribution of Bambusa ervariabilis × Dendrocalamopsis daii blight[J]. Journal of Sichuan Forestry Science and Technology, 2009, 30(1):29-31,37.
[4] 李潞滨, 李术娜, 李佳, 等. 毛竹枯梢病拮抗细菌分离鉴定及其拮抗物质[J]. 林业科学, 2009, 45(7):63-69. Li L B, Li S N, Li J, et al. Screening, identification and fermentation conditions optimization of antagonistic strain 6-59 of Bacillus metaterium against Ceratosphaeria phyllostachydis[J]. Scientia Silvae Sinicae, 2009, 45(7):63-69.
[5] Robert W B, Eloise K. Fungal pathogens of Miconia calvescens(Melastomataceae)from Brazil, with reference to classical biological contro1[J].Mycologia,2007,99(1):99-111.
[6] Gabricle B, Christin Z, Jana L, et a1. Impact of plant species and site on rhizosphere-associated fungi antagonistic to Verticillium daldiae Kleb[J]. Applied and Environmental Microbiology,2005,71(8):4203-4213.
[7] 余应建. 一株撑绿杂交竹内生菌的分离鉴定及主要培养特性研究[J]. 安徽农学通报, 2008, 14(17):64-67. Yu Y J. Study on isolation, identification and main cultural character of endophytic fungus in chenglv hybrid bamboo[J]. Anhui Agricultural Science Bulletin, 2008, 14(17):64-67.
[8] 李姝江,朱天辉,李芳莲,等. 杂交竹梢枯病拮抗细菌筛选及其抗菌物质研究[J]. 中国生物防治,2010, 26(S1):55-61. Li S J, Zhu T H, Li F L, et al. Selection of an antagonistic bacterium against Arthrinium phaeospermum and analysis of its antibacterial substance[J]. Chinese Journal of Biological Control, 2010, 26(S1):55-61.
[9] 方中达. 植病研究方法[M]. 北京:中国农业出版社, 1998.
[10] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京:科学出版社, 2001.
[11] 周德庆. 微生物学实验手册[M].上海:上海科技出版社, 1983.
[12] Kim S B, Yoon J H, Kim H, et a1. A phylogenetic analysis of the genus Saccharomonospora conducted with 16SrRNA gene sequences[J]. International Journal of Systematic Bacteriology, 1995,45(2):351-356.
[13] Rainey F A, Rainey N W, Kroppenstedt R M, et a1. The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage:Proposal of Nocardiopsaceae fam. nov.[J]. International Journal of Systematic Bacteriology, 1996, 46(4):1088-1092.
[14] 那广水, 张婷, 叶亮, 等. 北极放线菌AFN2001培养基和发酵条件的优化实验[J]. 海洋学研究, 2008, 26(4):66-71. Na G S, Zhang T, Ye L, et al. Optimization of the media and fermentation conditions of arctic actinomycete AFN2001[J]. Journal of Marine Sciences, 2008, 26(4):66-71.
[15] 曹煜成, 李卓佳, 吴灶和, 等. 地衣芽孢杆菌胞外蛋白酶的纯化及特性分析[J]. 水生生物学报, 2006, 30(3):262-268. Cao Y C, Li Z J, Wu Z H, et al. Purification and characterization of extracellular protease of Bacillus licheniformis[J]. Acta Hydrobiologica Sinica, 2006, 30(3):262-268.
[16] 梁漫. 生防芽孢杆菌的筛选及其抗菌物质的研究[D]. 雅安:四川农业大学,2011.
[17] 魏松红, 纪明山, 李艳丽, 等. 番茄叶霉病菌拮抗菌的筛选及其发酵条件[J]. 中国生物防治, 2007, 23(1):60-63. Wei S H, Ji M S, Li Y L, et al. Selection of antagonistic microorganisms against Fulvia fulva and analysis of fermentation conditions[J]. Chinese Journal of Biological Control, 2007, 23(1):60-63.
[18] 吴国江, 王占利, 李术娜, 等. 大丽轮枝菌拮抗细菌8-52菌株的筛选与鉴定[J]. 河北农业大学学报, 2009, 32(4):66-70. Wu G J, Wang Z L, Li S N, et al. Screening and identification of intagonistic bacterium strain 8-52 against Verticillium dahlia[J]. Journal of Agricultural University of Hebei, 2009, 32(4):66-70.
[19] 燕海潮, 曾来涛, 郭晓军, 等. 大丽轮枝菌颉颃细菌2-14菌株的鉴定[J]. 畜牧与饲料科学, 2009, 30(4):6-7. Yan H C, Ceng L T, Guo X J, et al. Identification of antagonistic bacterium strain 2-14 against Verticillium dahlia[J]. Animal Husbandry and Feed Science, 2009, 30(4):6-7.
[20] 王伟, 李术娜, 李红亚, 等. 大丽轮枝菌拮抗细菌菌株12-51的筛选鉴定与抗菌物性质分析[J].中国农学通报, 2009, 25(19):14-19. Wang W, Li S N, Li H Y, et al. Screening, identification of the antagonistic bacteria against Verticillium dahliae Kleb and characteristic analyses of antagonistic substance[J]. Chinese Agricultural Science Bulletin, 2009, 25(19):14-19.
[21] 何李黎. 地衣芽孢杆菌ZJU12所产细菌素的分离纯化及其理化性质和基因克隆的研究[D].杭州:浙江大学, 2006.
[22] 张翠竹, 张心平, 梁凤来, 等. 一株地衣芽孢杆菌产生的生物表面活性剂[J]. 南开大学学报:自然科学, 2000, 33(4):41-52. Zhang C Z, Zhang X P, Liang F L, et al. A biosurfactant produced by Bacillus licheniformis SNK-X3[J]. Acta Scientiarum Naturalium Universitatis Nankaiensis, 2000, 33(4):41-52.
[23] 黄现青, 别小妹, 吕凤霞, 等. 枯草芽孢杆菌fmbJ产脂肽抑制点青霉效果及其桃防腐试验[J]. 农业工程学报, 2008, 24(1):263-267. Huang X Q, Bie X M, Lv F X, et al. Inhibitory effect of lipopeptides from Bacillus subtilis fmbJ on Penicillium notatum and its antisepsis to peach[J]. Transactions of the CSAE, 2008, 24(1):263-267.
[24] 陈华,郑之明,余增量,等.枯草芽孢杆菌JA脂肽类及挥发性物质抑菌效应的研究[J]. 微生物学通报2008, 35(1):1-4. Chen H, Zheng Z M, Yu Z L, et al. Antagonistic properties of lipopeptides and volatiles produced by Bacillus subtilis JA[J]. Microbiology, 2008, 35(1):1-4.
[25] 纪兆林, 唐丽娟, 张清霞, 等. 地衣芽孢杆菌W10抗菌蛋白的分离纯化及其理化性质研究[J]. 植物病理学报, 2007, 37(3):260-264. Ji Z L, Tang L J, Zhang Q X, et al. Isolation, purification and characterization of antifungal protein from Bacillus licheniformis W10 strain[J]. Acta Phytopathologica Sinica, 2007, 37(3):260-264.
[26] 邢介帅, 李然, 赵蕾, 等. 生防芽孢杆菌T2胞外蛋白酶的纯化及其抗真菌作用[J]. 植物病理学报, 2008, 38(4):377-381. Xing J S, Li R, Zhao L, et al. Purification, characterization and antagonism of an extracellular protease from Bacillus subtilis strain T2[J]. Acta Phytopathologica Sinica, 2008, 38(4):377-381.
[27] 任争光, 宋庆丰, 张志勇, 等. 杏树根际芽孢杆菌BJ-6抗菌蛋白的分离纯化及抑菌机理[J]. 果树学报, 2009, 26(3):325-328. Ren Z G, Song Q F, Zhang Z Y, et al. Purification and mechanism of antifungal protein from Bacillus strain BJ-6[J]. Journal of Fruit Science, 2009, 26(3):325-328.

Last Update: 2013-11-30