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一株高温蛋白酶高产菌株产酶条件的优化(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2014年01期
Page:
31-35
Column:
研究论文
publishdate:
2014-02-16

Article Info:/Info

Title:
Optimization of fermentation medium and culture condition of a thermostable protease from moderate thermophilic strain Bacillus subtilis BY25
Article ID:
1000-2006(2014)01-0031-05
Author(s):
ZHU Hong123 WANG Yiming1LIN Xiangui1*
1.State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;
2.Institute of Botany, Jiangsu Province & Chinese Academy of Science, Nanjing 210018, China;
3.University of Chinese Academy of Sciences, Beijing 100049, China
Keywords:
Bacillus subtilis BY25 thermostable protease culture medium fermentation conditions
Classification number :
Q939.96
DOI:
10.3969/j.issn.1000-2006.2014.01.006
Document Code:
A
Abstract:
The fermentation media components and culture conditions of the thermostable protease from a thermophilic strain Bacillus subtilis BY25 were further studied. A six factor and three level orthogonal experiment was conducted to observe the effects of fermentation medium components on thermostable protease yield. Wheat bran was replaced by soybean cake powder for its low contribution rate. Single factor experiment was carried out to optimize the protease production. The optimized culture condition was at 30 ℃, with initiating pH of 8.0, 4.5%-5% inoculum size for 72 h; and the optimized fermentation medium components were described as follows:glucose 60 g/L, soybean cake powder 60 g/L, Na2HPO4 4 g/L and CaCl2 0.5 g/L. After fermentation, the casein hydrolysis activity of the enzyme was estimated up to 101.1 μmol/(min·mL), much higher than other domestic reports.

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Last Update: 2014-01-15