我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|Table of Contents|

枣实蝇及其他5种检疫性实蝇的PCR-RFLP快速鉴定(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2014年01期
Page:
183-186
Column:
研究简报
publishdate:
2014-02-16

Article Info:/Info

Title:
Rapid identification of Carpomya vesuviana Costa and five relative species of fruit flies by PCR-RFLP
Article ID:
1000-2006(2014)01-0183-03
Author(s):
CHENG Xiaotian12ADILI Sattar12*ZHANG Wei3LI Xinquan12
1.College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, China;
2.Key Laboratory of Forestry Ecology and Industry Technology in Arid Region, Education Department of Xinjiang, Xinjiang Agricultural University, Urumqi 830052, China;
3.Xinjiang Entry-Exit Inspection and Quarantine Bureau, Urumqi 830083, China
Keywords:
Carpomya vesuviana Costa quarantine fruit flies PCR-RFLP mtDNA
Classification number :
Q965
DOI:
10.3969/j.issn.1000-2006.2014.01.033
Document Code:
A
Abstract:
Carpomya vesuviana Costa has been a new quarantine pest in China. In this paper, the method of rapid identification of C. vesuviana Costa and 5 relative species of fruit flies was studied based on PCR-RFLP of the mitochondrial DNA(mtDNA). The results showed that DNAs, which were estimated about 350 bp and 450 bp respectively, were obtained by means of PCR with two pairs of primers designed from mtDNA of target pests.The PCR-products could be registered by restriction enzymes(Dra I and Mse I). All the 6 species could be discriminated according to the banding patterns of the restriction enzymes. It is suggested that the method is reliable for quick discrimination of fruit flies.

References

[1] 张润志,汪兴鉴,阿地力·沙塔尔.检疫性害虫枣实蝇的鉴定与入侵威胁[J].昆虫知识,2007,44(6):928-930.Zhang R Z, Wang X J, Adili S. Identification and precaution of the ber fruit fly[J]. Chinese Bulletin of Entomology,2007,44(6):928-930.
[2] 阿地力·沙塔尔,何善勇,田呈明,等.枣实蝇在吐鲁番地区的发生及蛹的分布规律[J].植物检疫,2008,22(5):295-297.Adili S,He S Y, Tian C M,et al. Distribution pattern of occurrence and pupae of Carpomya vesuviana Costa in the Turpan region [J]. Plant Quarantine, 2008,22(5):295-297.
[3] 吴佳教,胡学难,赵菊鹏,等.9种检疫性实蝇PCR-RFLP快速鉴定研究[J].植物检疫,2005,19(1):2-6.Wu J J,Hu X N, Zhao J P,et al. Rapid identification among 9 species of quarantine fruit flies(Diptera:Tephritidae)by PCR-RFLP[J].Plant Quarantine,2005,19(1):2-6.
[4] Muraji M,Nakahara S.Phylogenetic relationships among fruit flies,Bactrocera (Diptera:Tephritidae)based on the mitochondrial rDNA sequences [J].Insect Mol Biol,2001,10(6):549-559.
[5] Muraji M,Nakahara S.Discrimination among pest species of Bactrocera (Diptera:Tephritidae)based on PCR-RFLP of the mitochondrial DNA[J].Appl Entomol Zoo1,2002,37(3):437-446.
[6] 陈乃中.利用RAPD-PCR技术区分4种仓虫幼虫的初步研究[J].植物保护,1997,23(3):20-21.Chen N Z. Preliminary study using RAPD-PCR technique to distinguish four kinds of positions larvae[J].Plant Protection,1997,23(3):20-21.
[7] 宁红,秦蓁.分子生物学技术在检疫性有害生物诊断中的应用[J].植物检疫,2002,16(2):98-100.Ning H,Qin Z. The application of molecular biology techniques in the diagnosis of quarantine pests[J]. Plant Quarantine, 2002,16(2):98-100.
[8] 安榆林,朱宏斌,焦国尧.RAPD-PCR及其在检疫性害虫鉴定中的应用[J].中国进出境动植检,1997,(2)38-39.An Y L,Zhu H B,Jiao G Y. RAPD-PCR and quarantine pests identified[J]. China’s entry and exit phytosanitary,1997,(2)38-39.
[9] 陈萍,温硕洋,曾玲. PCR-RFLP技术应用于鉴定美洲斑潜蝇和番茄斑潜蝇的初步研究[J]. 武夷科学, 2002, 18(6):60-64.Chen P,Wen S Y,Zeng L. A study on Liriomyza sativae and Liriomyza bryoniae by PCR-RFLP[J]. Wuyi Science Journal, 2002,18(6):60-64.
[10] 吴佳教,梁帆,胡学难,等.我国南方常见的6种寡毛实蝇PCR-RFLP快速鉴定研究[J].江西农业大学学报,2004, 26(5):770-773.Wu J J,Liang F, Hu X N,et al. A study on quick discrimination among 6 species of fruit flies(Tephritidae:Bactrocera)in South China based on PCR—RFLP[J]. Acta Agricuhurae Universitatis Jiangxiensi,2004,26(5):770-773.
[11] 梁广勤.实蝇[M]. 北京:中国农业出版社,2011.
[12] 叶军,周国梁,易建平,等. 地中海实蝇幼虫分子鉴定[J].昆虫知识,2007,44(4):562-566.Ye J, Zhou G L,Yi J P,et al.Molecular identificationfor larvae of Ceratitis capitata[J].Chinese Bulletin of Entomology, 2007,44(4):562-566.
[13] 李志红,梁广勤,梁帆,等.GIS与实蝇监测[J].江西农业大学学报,2002,24(6):401-405.Li Z H, Liang G Q, Liang F,et al. GIS Technology and fruit fly monitoring[J].Acta Agriculturae Universitis Jiangxiensis, 2002,24(6):401-405.
[14] 袁盛勇,肖春,李正跃,等.桔小实蝇实验室饲养技术研究[J].江西农业大学学报,2003,25(4):577-580.Yuan S Y,Xiao C,Li Z Y,et al.A study on laboratory rearing techniques for Bactrocera dorsalis(Hendal)[J].Acta Agriculturae Universitis Jiangxiensis, 2003,25(4):577-580.
[15] Armstrong K F, Cameron C M, Frampton E R. Fruit fly(Diptera:Tephritidae)species identification:a rapid molecular diagnostic technique for quarantine application[J]. Bulletin of Entomological Research, 1997, 87(2):111-118.

Last Update: 2014-01-15