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基于松材线虫全基因组序列的SSR标记开发(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2014年02期
Page:
36-42
Column:
研究论文
publishdate:
2014-03-24

Article Info:/Info

Title:
Development of simple sequence repeats base on pine wood nematode (Bursaphelenchus xylophilus)genome sequence
Article ID:
1000-2006(2014)02-0036-07
Author(s):
XU Junrong WU Xiaoqin* LIU Yun YE Jianren
Jiangsu Key Laboratory for Prevention and Management of Invasive Species,College of Forest Resources and Enviroment,Nanjing Forestry University,Nanjing 210037,China
Keywords:
Bursaphelenchus xylophilus whole genome sequences simple sequence repeat(SSR) genetic polymorphism
Classification number :
Q78; S763.16
DOI:
10.3969/j.issn.1000-2006.2014.02.008
Document Code:
A
Abstract:
To develop more stable molecular marker, SSR marker base on pine wood nematode(Bursaphelenchus xylophilus)whole genome sequences was discussed for researching the relationship of population genetic and travel route accurately. Ninety-five SSR loci were searched from a total of 10 432 DNA fragments of B.xylophilus by MISA. In the gSSRs, the dinucleotide repeat motifs were the most abundant(66.3%). Thirty-six pairs of primers were designed, and verified with one peace of B.xylophilus DNA pooling sample(46 DNA samples of isolates from different geographic origin contained)for PCR. The products detected by QIAxcel automatic gel electrophoresis analysis system showed that 17 pairs of primers had potential polymorphism. Further, nine pairs of the primers showed polymorphism, which tested by monoclonal sequencing and spliced by BioEdit, and which provided foundation for studying population genetic of B.xylophilus.

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Last Update: 2014-03-10