我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|Table of Contents|

毛竹PeSCL6基因的克隆及其表达分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2014年02期
Page:
43-46
Column:
研究论文
publishdate:
2014-03-24

Article Info:/Info

Title:
Cloning and expression analysis of PeSCL6 gene in Phyllostachys edulis Carr.
Article ID:
1000-2006(2014)02-0043-04
Author(s):
CHEN Dongliang12 PENG Zhenhua13 GAO Zhimin1*
1. International Center for Bamboo and Rattan, Key Laboratory on the Science and Technology of Bamboo and Rattan, SFA, Beijing 100102, China;
2. Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijin
Keywords:
Phyllostachys edulis Carr. PeSCL6 real time PCR
Classification number :
Q78; S795
DOI:
10.3969/j.issn.1000-2006.2014.02.009
Document Code:
A
Abstract:
SCL6 is one of the key genes for plant to maintain the shoot apical meristem tissue in indeterminate state. A homologue gene of SCL6 was cloned from moso bamboo(Phyllostachys edulis Carr.)using RT-PCR and RACE methods, and named as PeSCL6. The full length of PeSCL6 was 1 894 bp including 60 bp 5' untranslated region(UTR), 211 bp 3' UTR and an open reading frame of 1 623 bp, which encoded 489 amino acids. Blastp analysis indicated that PeSCL6 had high identities with SCL6 from monocotyledon plants(all up to more than 70%)such as Zea mays, Oryza sativa and Sorghum bicolor. Real time PCR analysis showed that PeSCL6 expressed constitutively with the highest level in leaves of young seedlings. However, the PeSCL6 expressed much lower in the leaves at adult bamboo at just before and full blooming stages, which were only 1% and 14% of that in young seedling leaves, respectively. The changes of expression level in leaves indicated that PeSCL6 gene might be involved in the switch regulation of moso bamboo from vegetative to reproductive growth.

References

[1] Lee M H, Kim B, Song S K, et al. Large-scale analysis of the GRAS gene family in Arabidopsis thaliana[J]. Plant Molecular Biology, 2008, 67(6): 659-670.
[2] Engstrom E M, Andersen C M, Gumulak-Smith J, et al. Arabidopsis homologs of the petunia hairy meristem gene are required for maintenance of shoot and root indeterminacy[J]. Plant Physiology, 2011, 155(2): 735-750.
[3] Stuurman J, Jäggi F, Kuhlemeier C. Shoot meristemmaintenance is controlled by a GRAS-gene mediated signal from differentiating cells[J]. Genes Development, 2002, 16(17): 2213-2218.
[4] Schulze S, Schäfer B N, Parizotto E A, et al. LOST MERISTEMS genes regulate cell differentiation of central zone descendants in Arabidopsis shoot meristems[J]. Plant Journal, 2010, 64(4): 668-678.
[5] Wang L, Mai Y X, Zhang Y C, et al. MicroRNA171c-targeted SCL6-Ⅱ, SCL6-Ⅲ, and SCL6-IV genes regulate shoot branching in Arabidopsis[J]. Molecular Plant, 2010, 3(5): 794-806.
[6] 熊文愈,丁祖福,李又芬.竹类植物的居间分生组织与节间生长Ⅰ秆茎的居间分生组织与节间生长[J]. 林业科学,1980,16(2):81-89. Xiong W Y, Ding Z F, Li Y F. Intercalary meristem and internodal elongation of bamboo plants[J].Scientia Silvae Sinicae, 1980, 16(2): 81-89.
[7] 吴晓宇,胡尚连,曹颖,等. 慈竹CCoAOMT基因的克隆及生物信息学分析[J]. 南京林业大学学报:自然科学版, 2012, 36(3):17-22. Wu X Y, Hu S L, Cao Y, et al. Cloning of CCoAOMT gene in Neosinocalamus affinis and its bioinformatics analysis[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2012, 36(3):17-22.
[8] Gao Z M, Li X P, Li L L, et al. An effective method for total RNA isolation from bamboo[J]. Chinese Forestry Science and Technology, 2006, 5(3): 52-54.
[9] 高志民,彭镇华,李雪平,等.毛竹苯丙氨酸解氨酶基因的克隆及组织特异性表达分析[J]. 林业科学研究,2009,22(3):449-453. Gao Z M, Peng Z H, Li X P, et al. Isolation and tissue specific expression analysis of phenylanlanine ammonialyase gene from Phyllostachys edulis[J]. Forest Research, 2009, 22(3): 449-453.
[10] Livak K J, Schmittgen D T. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method[J]. Methods, 2001, 25(4): 402-408.
[11] Pysh D L, Wysocka-Diller J W,Camilleri C, et al. The GRAS gene family in Arabidopsis: sequence characterization and basic expression analysis of the SCARECROW-LIKE genes[J].The Plant Journal, 1999, 18(1): 111-119.
[12] Bolle C, Koncz C, Chua N H. PAT1, a new member of the GRAS family, is involved in phytochrome A signal transduction[J]. Genes Development, 2000, 14(10): 1269-1278.
[13] Brand U, Fletcher J C, Hobe M, et al. Dependence of stem cell fate in Arabidopsis on a feedback loop regulated by CLV3 activity[J]. Science, 2000, 289: 617-619.
[14] Schoof H, Lenhard M, Haecker A, et al. The stem cell population of Arabidopsis shoot meristems is maintained by a regulatory loop between the CLAVATA and WUSCHEL genes[J]. Cell, 2000, 100(6): 635-644.
[15] Long J A, Moan E I, Medford J I, et al. A member of the KNOTTED class of homeodomain proteins encoded by the STM gene of Arabidopsis[J]. Nature, 1996, 379: 66-69.
[16] Eric M E. HAM proteins promote organ indeterminacy: but how?[J]. Plant Signaling and Behavior, 2012, 7(2): 227-234.
[17] Mi-Hyun L, Bohye K, Sang-Kee S, et al. Large-scale analysis of the GRAS gene family in Arabidopsis thaliana[J]. Plant Molecular Biology, 2008, 67(6): 659-670.

Last Update: 2014-03-10