我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|Table of Contents|

造纸白水中的细菌多样性及系统发育树分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2014年02期
Page:
123-127
Column:
研究论文
publishdate:
2014-03-24

Article Info:/Info

Title:
Bacterial diversity and phylogenetic analysis of whitewater in papermaking process
Article ID:
1000-2006(2014)02-0123-05
Author(s):
WU Fangfang1CHENG Qiang2SHI Liuxin3*
1.College of Chemical Engineering, Nanjing Forestry University,Nanjing 210037,China;
2.Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement, Nanjing Forestry University, Nanjing 210037, China;
3.College of Light Indu
Keywords:
paper machine whitewater 16S rDNA bacterial diversity Schlegelella aquatica
Classification number :
TS75; Q19
DOI:
10.3969/j.issn.1000-2006.2014.02.023
Document Code:
A
Abstract:
Paper machines offer a suitable environment for microbial growth, which results in slime problems during the process of papermaking. Investigation of the bacterial diversity in whitewater which recycled in paper mill UPM was carried out in this paper. Total DNA were directly extracted from the whitewater sample. 16S rDNA were amplified directly from purified DNA by PCR with universally bacteria-specific rDNA primers and cloned. Sixty-one bacteria were identified by partial sequencing of their 16S rDNAs. Twenty-eight operational taxonomic units(OTUs)were clustered in the following phyla including Proteobacteria(77.05%), Firmicutes(11.48%), Actinobacteria(4.92%), Deinococcus-Thermus(3.28%), Bacteroidetes(1.64%),Cyanobacteria(1.64%). Bacterial diversity was high in the whitewater sample. The results showed a high proportion of Schlegelella aquatica in the white water samples. It was the first time that Schlegelella aquatica strain had been identified from a paper machine in China. We also identified the red-slime formation bacteria Deinococcus-Thermus in whitewater sample.

References

[1] 黄淋佳,黄创锋,杨伟和,等. 造纸沉积物的产生和防治[J].广东化工, 2009, 36(7):306-307. Huan L J, Huang C F,Yang W H, et al.Prevention and control of deposits in papermaking process[J].Guangdong Chemical Industry,2009, 36(7):306-307.
[2] Blanco M A, Negro C, Gaspar I, et al. Slime problems in the paper and board industry[J]. Appl Microbiol Biotechnol, 1996, 46(3):203-208.
[3] Lacorte S, Latorre A, Barceló D, et al. Organic compounds in paper-mill process waters and effluents[J]. TrAC Trends in Analytical Chemistry, 2003, 22(10):725-737.
[4] Lahtinen T, Kosonen M, Tiirola M, et al. Diversity of bacteria contaminating paper machines[J]. Journal of Industrial Microbiology and Biotechnology, 2006, 33(9):734-740.
[5] Desjardins E, Beaulieu C. Identification of bacteria contaminating pulp and a paper machine in a Canadian paper mill[J]. Journal of Industrial Microbiology and Biotechnology, 2003, 30(3):141-145.
[6] Ekman J. Bacteria colonizing paper machines[D]. Helsinki:Department of Food and Environmental Sciences Faculty of Agriculture and ForestryUniversity of Helsinki, 2011.
[7] 赵丰,焦虎美,时留新.造纸网下白水所含细菌的分离和鉴定[J].南京林业大学学报:自然科学版, 2011,35(3):115-119. Zhao F, Jiao H M, Shi L X.Separation and identification of microorganisms from the white water in the wire pit[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2011,35(3):115-119.
[8] 焦虎美, 时留新.腐浆中细菌的鉴定及其生物膜活性研究[J]. 南京林业大学学报:自然科学版,2012,36(2):77-81. Jiao H M, Shi L X.Identification of bacterias from the slime and the study on their biofilm activity[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2012,36(2):77-81.
[9] Pace N R. A molecular view of microbial diversity and the biosphere[J]. Science,1997,5313: 734-740.
[10] 国家环境保护总局.水和废水监测分析方法[M]. 4版. 北京:中国环境科学出版社, 2002.
[11] Zhou J, Bruns M A, Tiedje J M. DNA recovery from soils of diverse composition[J]. Applied and Environmental Microbiology, 1996, 62(2):316-322.
[12] Granhall U, Welsh A, Throbäck I N, et al. Bacterial community diversity in paper mills processing recycled paper[J]. Journal of Industrial Microbiology & Biotechnology, 2010, 37(10):1061-1069.
[13] Chou Y J, Sheu S Y, Sheu D S, et al. Schlegelella aquatica sp. nov., a novel thermophilic bacterium isolated from a hot spring[J]. International Journal of Systematic and Evolutionary Microbiology, 2006, 56(12):2793-2797.
[14] Kolari M, Nuutinen J, Rainey F A, et al. Colored moderately thermophilic bacteria in paper-machine biofilms[J]. Journal of Industrial Microbiology and Biotechnology, 2003, 30(4):225-238.
[15] Julie D, Beaulieu C, Villemur R. Composition of the bacterial biota in slime developed in two machines at a Canadian paper mill[J]. Canadian Journal of Microbiology, 2011, 57(2):91-104.
[16] Prince V, Simao-Beaunoir A M, Beaulieu C. Amplified ribosomal DNA restriction analysis of free-living bacteria present in the headbox of a Canadian paper machine[J]. Canadian Journal of Microbiology, 2009, 55(7):810-817.
[17] 董玉玲,朱万斌,郭鹏,等.一组小麦秸秆好氧分解菌系的构建及组成多样性[J]. 环境科学, 2010, 31(1):249-254. Dong Y L,Zhu W B,Guo P, et al. Characteristics and diversity of a microbial community decomposing wheat straw under aerobic conditions[J].Environmental Science,2010, 31(1):249-254.

Last Update: 2014-03-10