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白桦BpMYB2基因及其启动子克隆、表达分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2014年03期
Page:
24-28
Column:
研究论文
publishdate:
2014-05-15

Article Info:/Info

Title:
Cloning and expression analysis of the BpMYB2 gene and its promoter in Betula platyphylla
Article ID:
1000-2006(2014)03-0024-05
Author(s):
ZHANG NanCUI ZhiyuanSUN DanWANG Chao*
State Key Laboratory of Forest Tree Genetic Improvement and Biotechnology, Northeast Forestry University, Harbin 150040,China
Keywords:
BpMYB2 promoter gene expression Betula platyphylla
Classification number :
Q781; S722
DOI:
10.3969/j.issn.1000-2006.2014.03.005
Document Code:
A
Abstract:
In this study, a MYB transcription factor named BpMYB2 was cloned from Betula platyphylla. The realtime PCR analysis showed that the expression of BpMYB2 was various in different tissues, and it expressed in the highest level in normal wood, then the second level in opposite wood, and then in tension wood and flower, while the least expression were in leaves and buds. A 1 284 bp BpMYB2 promoter was cloned by genome walking method, and the promoter included some cis-elements such as TATA box, ARR1AT, WRKY71OS by PLACE analysis. Further, the BpMYB2 promoter was inserted to pCAMBIA1301 vector under control of the 35S promoter to generate the BpMYB2-1301 recombinant construct, which was transient expressed in B. platyphylla seedlings by Agrobacterium tumefaciens mediated method. The histochemical GUS staining showed that the BpMYB2 promoter could drive the expression of GUS gene in B. platyphylla, and expressed in high level in stems and petioles while in low level in leaves. In conclusion, BpMYB2 is important forxylem development and lay basis for utilizing the transcription factor to regulate the xylem development in B. platyphylla.

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Last Update: 2014-05-15