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|Table of Contents|

杨树MAGPIEJACKDAW基因克隆、表达及蛋白互作研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2014年03期
Page:
29-34
Column:
研究论文
publishdate:
2014-05-15

Article Info:/Info

Title:
Cloning, expression and interaction analysis of MAGPIE and JACKDAW genes in Populus
Article ID:
1000-2006(2014)03-0029-06
Author(s):
XUAN Lei XU Meng XU Li’an HUANG Minren*
College of Forest Resource and Environment,Nanjing Forestry University,Nanjing 210037,China
Keywords:
adventitious root of Populus expression pattern of gene subcellular localization bimolecular fluorescence complementation(BiFC)
Classification number :
Q781; S722
DOI:
10.3969/j.issn.1000-2006.2014.03.006
Document Code:
A
Abstract:
The PeMGP and PeJDK genes were cloned from adventitious roots of Populus, and their full-length cDNA sequences contain open reading frame(ORF)of 1 434 bp and 1 518 bp, respectively. There were two introns in their genomic structure. The two deduced proteins all contained 4 identical zinc finger domains, ZF1(CX2-4CX3FX4LX2HX3-5H)and ZF2(CX4CXnHXnX4H), ZF3(CX2CXnHX3C)and ZF4(CXCXnHX3C). Quantitative real-time PCR analysis revealed that two genes expressed in different organs, and showed a dynamic expression pattern in Populus adventitious roots. Transient gene expression assay in Populus mesophyll protoplasts demonstrated that PeMGP and PeJDK were localized in nucleus, and their protein interact each other in nucleus.

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Last Update: 2014-05-15