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低温胁迫下梁山慈竹再生植株叶绿素荧光特性和耐寒转录因子的表达(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2014年04期
Page:
39-44
Column:
研究论文
publishdate:
2014-07-01

Article Info:/Info

Title:
Chlorophyll fluorescence parameters and expression of cold-related transcription factors in regenerated plants of Dendrocalamus farinosus under cold stress
Article ID:
1000-2006(2014)04-0039-06
Author(s):
CHEN Rong ZHANG Li CAO Ying LU Xueqin HU Shanglian* DUAN Ning
College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
Keywords:
Dendrocalamus farinosus regenerated plants cold stress chlorophyll fluorescence parameter transcription factor
Classification number :
S718.46
DOI:
10.3969/j.issn.1000-2006.2014.04.008
Document Code:
A
Abstract:
In order to explore the cold-resistance of the regenerated plants from Dendrocalamus farinosus, the seedlings of D. farinosus(SS)and three regenerated plants(No.101-1c, No.42-1-B and No.90-3)induced from D. farinosus mature embryo in vitro were exposed to cold stress in this study. Their chlorophyll fluorescence parameters and expression level of cold-related transcription factors under cold stress were investigated. It aimed to the results showed that SS could be resistant to 0 ℃; No.90-3 could be resistant to 4 ℃,while No.101-1c and No.42-1-B could be resistant to-10 ℃. It indicated that the cold-resistance of three regenerated plants was different and had obvious difference with SS. Under cold stress, Fv/Fm, Y(Ⅱ)and qP of SS, No.101-1c and No.90-3 decreased with the temperature decreasing, while Y(Ⅱ) of No.42-1-B went up and then descended, but the Fv/Fm, NPQ and qP reduced. The expression level of WRKY and CBF1 from SS decreased, while the expression level of MYB increased. In No.90-3 and No.42-1-B, the increase of expression level of MYB, WRKY and CBF1 was observed, and the transcription accumulation of WRKY and CBF1 from No.101-1c increased, but the expression level of MYB reduced under the cold stress. The results of correlation analysis showed that the expression of WRKY and CBF1 from SS had a significant positive relationship with Y(Ⅱ); the relationship between MYB and Y(Ⅱ)from No.101-1c was positive significantly, while there was a significant negative relationship among NPQ, WRKY and CBF1. In addition, WRKY showed a significant negative relationship to qP of No.42-1-B.

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Last Update: 2014-07-31