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木聚糖酶XynB分子Ser/Thr 平面导入精氨酸对酶热稳定性的影响(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2014年04期
Page:
107-112
Column:
研究论文
publishdate:
2014-07-01

Article Info:/Info

Title:
Thermostability of xylanase(XynB)by introducing Arg into its Ser/Thr surface
Article ID:
1000-2006(2014)04-0107-06
Author(s):
LI Fei12 XIE Jingcong1LI Qi1ZHANG Xuesong4ZHAO Linguo13*
1.College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China;
2.College of Biochemical Engineering, Taizhou College, Nanjing Normal University, Taizhou 225300, China;
3.Jiangsu Key Lab of Biomass Based Green Fuels and Chemicals, Nanjing 210037, China;
4.Department of Biological Engineering, Jiangsu Polytechnic College of Agriculture and Forestry, Zhenjiang 212400, China
Keywords:
site-directed mutagenesis xylanase arginine thermostability
Classification number :
TQ936
DOI:
10.3969/j.issn.1000-2006.2014.04.020
Document Code:
A
Abstract:
Replacing several serine and threonine residues in the Ser/Thr surface of xylanase(XynB)with arginines effectively increased the thermostability of the enzyme by site-directed mutagenesis. The mutated enzymes XynB-104 and XynB-77 were expressed in Pichia pastoris and their enzymatic properties were determined. The optimal temperature of the mutations XynB-104 and XynB-77 were 50 ℃, and the relative activity of mutations were dramatically increased. Both mutated xylanases showed about 80% of maximal activity after incubating in xylan substrate for 2 h at 50 ℃ compared to only 26% activity of the native enzyme. The optimal pH of XynB-104 enzyme had no change, but the pH optimum of the XynB-77 enzyme was increased from 5.0 to 5.5. The results of the mutated enzymes indicated that properly arginines residues introduced into Ser/Thr surface of xylanase XynB might be effective to improve the enzyme thermostability and hydrolysis capacity.

References

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Last Update: 2014-07-31