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麦草碱性亚硫酸盐制浆废液的木聚糖酶解特性(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2014年04期
Page:
113-117
Column:
研究论文
publishdate:
2014-07-01

Article Info:/Info

Title:
Enzymatic hydrolysis characteristics of xylan in spent liquor of wheat straw alkaline sulfite
Article ID:
1000-2006(2014)04-0113-05
Author(s):
HOU Guangbei1FENG Nianjie1ZHANG Lin1DAI Kai1WANG Qi1ZHAI Huamin1*XUE Xihua2
1. College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037, China;
2. Nantong Shanye Industrial Co., Ltd., Nantong 226571, China
Keywords:
wheat straw alkaline sulfite pulping(ASP) spent liquor xylanase enzymolysis
Classification number :
TS745
DOI:
10.3969/j.issn.1000-2006.2014.04.021
Document Code:
A
Abstract:
The wheat straw alkaline sulfite pulping(ASP)is hopeful to be a potential non-wood lignocelluloses biomass biorefinery process. However, ASP spent liquor utilization is a bottleneck towards the biorefinery. In the paper, the enzymolysis conditions and properties of three kinds of xylanases from Bacillus alkalophilus, Trichoderma reacei and Pulpzyme H C were investigated based on the relative polysaccharide hydrolysis rate(RPHR). The results indicated that 27.7% of the RPHR was obtained by the xylanase hydrolysis of Bacillus alkalophilus at 6.0 μmol/(mL·min)of enzyme dosage, 50% of spent liquor concentration(weight percentage), pH 7.0, 50 ℃ for 8 h. 48.5% of the RPHR was found by the enzymolysis of Trichoderma reesei at 10.0 μmol/(mL·min)of enzyme dosage, 50% of spent liquor concentration, pH 5.0, 50 ℃ for 8 h. The RPHR of Pulpzyme HC xylanase reached to 48.0% at 8.33 μmol/(mL·min)of enzyme dosage, 50% of spent liquor concentration, pH 8.0, 55 ℃ for 4 h. The reducing sugar content of ASP spent liquor reached to 11.1 g/L from 1.2 g/L by the enzymolysis of Pulpzyme HC. Pulpzyme HC was more powerful and popular comparing with Bacillus alkalophilus and Trichoderma reesei in aspects of the efficiency and industrial application conditions. The enzymolysis of polysaccharide in ASP spent liquor was benefit to the following lignosulphonate separation and utlization.

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Last Update: 2014-07-31